Chemifluorescent substrate for peroxide and peroxidase detection
A kit for enhanced detection of analytes that produce peroxide or peroxidase activity.
1. A kit for detecting analytes that produce peroxide or exhibit peroxidase activity, the kit comprising
a resorufin derivative 10-acetyl-3,7-dihydroxyphenoxazine (ADHP),
a chemical enhancer sodium phenothiazine 10-yl propane sulfonate (NaPT),
an organic base catalyst dimethylaminopyridine (DMAP) and/or a 2-methyl imidazole,
a peroxide source, and
a buffer solution,
wherein the kit provides reagents for the detection of analytes that produce peroxide or peroxide activity.
2. The kit of claim 1 further comprising at least one of horseradish peroxidase (HRP), HRP conjugated streptavidin, or HRP conjugated antibodies.
3. The kit of claim 1 wherein the peroxide source is selected from the group consisting of hydrogen peroxide, urea peroxide, perborate and combinations thereof.
4. The kit of claim 1 wherein the buffer is selected from the group consisting of tris buffer saline, phosphate buffered saline, and combinations thereof.