Human parvovirus: bocavirus
Provided herein are sequences of the genomes and encoded proteins of a new human parvovirus, Bocavirus-2, and variants thereof. Also provided are methods of detecting the Bocavirus-2 and diagnosing Bocavirus-2 infection, methods of treating or preventing Bocavirus-2 infection, and methods for identifying anti-Bocavirus-2 compounds.
1. An isolated nucleic acid molecule comprising a nucleotide sequence having at least 80% identity to SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:43, SEQ ID NO:49, or a complement thereof.
2. An isolated nucleic acid molecule comprising a nucleotide sequence hybridizes under highly stringent conditions over the full length of SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:43, SEQ ID NO:49, or a complement thereof, wherein the hybridization reaction is incubated at 42° C. in a solution comprising 50% formamide, 5×SSC, and 1% SDS and washed at 65° C. in a solution comprising 0.2×SSC and 0.1% SDS.
3. The nucleic acid molecule of claim 1 , wherein the nucleotide sequence is at least 85% identical to SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:43, SEQ ID NO:49, or a complement thereof.
4. The nucleic acid molecule of claim 1 , wherein the nucleotide sequence is at least 90identical to SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:43, SEQ ID NO:49, or a complement thereof.
5. The nucleic acid molecule of claim 1 , wherein the nucleotide sequence is at least 95% identical to SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:43, SEQ ID NO:49, or a complement thereof.
6. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:43, SEQ ID NO:49, or a complement thereof.
7. A composition comprising a nucleic acid molecule of claim 1 .
8. An isolated Bocavirus comprising a nucleic acid molecule of claim 1 .
9. An expression vector comprising a nucleic acid molecule of claim 1 .
10. An isolated host cell comprising the expression vector of claim 9 .
11. A method of detecting a Bocavirus nucleic acid comprising:
a) contacting a sample suspected of containing a Bocavirus nucleic acid with a nucleotide sequence that hybridizes under highly stringent conditions to a nucleotide sequence of SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:43, SEQ ID NO:49, or a complement thereof; and
b) detecting the presence or absence of hybridization wherein the highly stringent conditions comprise an incubation at 42° C. in a solution comprising 50% formamide, 5X SSC, and 1% SDS and an washing at 65° C. in a solution comprising 0.2X SSC and 0.1% SDS.