Apoptosis modulator BCL-B and methods for making and using same
View Patent ↗A novel human member of the Bcl-2 family Bcl-B has been identified, which is closest in amino-acid sequence homology to the Boo (Diva) protein. The Bcl-B protein is widely expressed in adult human tissues. The Bcl-B protein modulates apoptosis. Bcl-B also binds Bcl-2. BCl-XL, and Bax but not Bak. Bcl-B displays a unique pattern of selectivity for binding and regulating the function of other members of the Bcl-2 family. Accordingly, provided herein is a method for identifying a molecule that binds to the Bcl-B polypeptide, by contacting the Bcl-B polypeptide with a test molecule and determining whether the test molecule binds to the polypeptide.
1. A method for identifying a molecule that binds to a polypeptide having at least about 99% identity to SEQ ID NO:2, said method comprising contacting the polypeptide with a test molecule and determining whether the test molecule binds to the polypeptide.
2. The method of claim 1 , wherein the polypeptide contains one or more 15N-labeled amino acids, and the binding is detected by resonance changes in the polypeptide.
3. The method of claim 1 , wherein the test molecule comprises a polypeptide sequence.
4. The method of claim 1 , wherein the polypeptide sequence comprises an antibody.
5. The method of claim 1 , wherein the test molecule is attached to the surface of a substrate.
6. The method of claim 1 , wherein the test molecule is in a library of molecules.
7. The method of claim 6 , wherein the library is attached at discrete positions of a substrate.
8. The method of claim 1 , wherein the contacting is in solution.
9. The method of claim 1 , wherein the polypeptide has the sequence of SEQ ID NO:2.
10. The method of claim 1 , wherein the polypeptide consists of the sequence of SEQ ID NO:2.
11. The method of claim 1 , wherein the polypeptide is attached to the surface of a substrate.
12. The method of claim 1 , wherein the contacting is in solid phase.
13. The method of claim 1 , wherein the contacting is in a cell.
14. The method of claim 1 , wherein the contacting is in situ.