Preparation of solvent-borne polymeric bioactive coatings
Processes for preparation of a protein-polymer composite material are provided according to embodiments of the present invention which include providing an admixture of a polymer resin, a surfactant and a non-aqueous organic solvent. An aqueous solution containing bioactive proteins and substantially free of surfactant is mixed with the admixture. The emulsion is mixed with a crosslinker to produce a curable composition. The curable composition is cured, thereby producing the protein-polymer composite material.
1. A process for preparation of a protein-polymer composite material, comprising:
providing an admixture of a polymer resin, a surfactant and a non-aqueous organic solvent;
mixing an aqueous solution containing bioactive proteins with the admixture, wherein the aqueous solution is substantially free of surfactant, to produce an emulsion
mixing the emulsion with a crosslinker to produce a curable composition; and
curing the curable composition, thereby producing the protein-polymer composite material.
2. The process of claim 1 , wherein the polymer resin is a hydroxyl-functionalized acrylate resin.
3. The process of claim 2 , wherein the crosslinker is a polyisocyanate.
4. The process of claim 1 , wherein the bioactive protein is an enzyme.
5. The process of claim 1 , wherein the bioactive protein is selected from the group consisting of: a lectin, an antibody and a receptor.
6. The process of claim 1 , further comprising addition of one or more additives to at least one of: the admixture, the aqueous solution, the emulsion, and the curable composition.
7. The process of claim 1 wherein the average particle size of bioactive protein particles in the protein-polymer composite material is in the range of 1 nm to 10 μm (average diameter), inclusive.
8. A process for preparation of a protein-polymer composite material, comprising:
providing an admixture of a hydroxyl-functionalized acrylate resin, a surfactant and a solvent, the solvent having a log P in the range of −0.5-2, inclusive;
mixing an aqueous solution containing a bioactive protein with the admixture, wherein the aqueous solution is substantially free of surfactant, to produce an emulsion;
mixing the emulsion with a polyisocyanate crosslinker to produce a curable composition; and
curing the polymerizable composition, thereby producing the composite material.
9. The process of claim 8 , wherein the bioactive protein is an enzyme.
10. The process of claim 8 , wherein the bioactive protein is selected from the group consisting of: a lectin, an antibody and a receptor.
11. The process of claim 8 , further comprising addition of one or more additives to at least one of: the admixture, the aqueous solution, the emulsion, and the curable composition.
12. The process of claim 8 wherein the average particle size of bioactive protein particles in the protein-polymer composite material is in the range of 1 nm to 10 μm (average diameter), inclusive.
13. The process of claim 1 , further comprising applying the curable composition to a substrate prior to curing the curable composition.
14. A process for preparation of a protein-polymer composite material, comprising:
providing an admixture of a polymer resin and a non-aqueous organic solvent;
mixing an aqueous solution containing bioactive proteins with the admixture, wherein the aqueous solution is substantially free of surfactant, to produce a first component;
providing a second component comprising a crosslinker;
mixing the first component and the second component to produce a curable composition; and
curing the curable composition, thereby producing the protein-polymer composite material.
15. The process of claim 14 , wherein the curing comprises thermal curing.
16. The process of claim 14 , wherein the curing comprises curing using actinic radiation.
17. The process of claim 14 , wherein the bioactive protein is an enzyme.
18. The process of claim 14 , wherein the bioactive protein is selected from the group consisting of: a lectin, an antibody and a receptor.
19. The process of claim 14 , further comprising addition of one or more additives to at least one of: the admixture, the aqueous solution, the emulsion, and the curable composition.
20. The process of claim 14 wherein the average particle size of bioactive protein particles in the protein-polymer composite material is in the range of 1 nm to 10 μm (average diameter), inclusive.
21. The process of claim 14 , further comprising applying the curable composition to a substrate prior to curing the curable composition.
22. A protein-polymer composite material, comprising:
bioactive proteins dispersed in a two component solvent-borne polymer resin, the average particle size of bioactive protein particles in the protein-polymer composite material is in the range of 1 nm to 10 μm (average diameter), inclusive, with the proviso that the bioactive proteins are not ion-paired.
23. A curable protein-polymer composite material, comprising:
bioactive proteins dispersed in a curable two component solvent-borne polymer resin, the average particle size of bioactive protein particles in the protein-polymer composite material is in the range of 1 nm to 10 μm (average diameter), inclusive, with the proviso that the bioactive proteins are not ion-paired.