MicroRNA biomarkers for human breast and lung cancer
View Patent ↗The present invention relates to novel molecular markers for diagnosis and classification of human breast cancer and lung cancer.
1. A pharmaceutical composition comprising
a) an oligonucleotide that down-regulates the over-expression of the miRNA or pre-miRNA of SEQ ID NO:25 in lung cancer, wherein said oligonucleotide is:
i) complementary to the nucleotide sequence of SEQ ID NO:25, or
ii) hybridizes under stringent conditions to a nucleotide sequence of i) or the nucleotide sequence of SEQ ID NO:25; and
b) a second active ingredient for prophylactic or therapeutic treatment of lung cancer.
2. The pharmaceutical composition of claim 1 , wherein the oligonucleotide is chemically modified.
3. A method for treating, preventing or reducing the risk of lung cancer associated with aberrant expression of a pre-miRNA and/or miRNA in a cell, tissue or animal comprising contacting the cell, tissue or animal with the pharmaceutical composition according to claim 1 .
4. The method of claim 3 , wherein the pharmaceutical composition is administered to an individual in need in a therapeutically effective amount.
5. The method of claim 4 , wherein the animal is a human being.
6. The method of claim 3 , wherein the oligonucleotide is administered by pulmonary administration.
7. The method of claim 3 , where the aberrant expression is up-regulation of a miRNA.
8. The method of claim 7 , where the oligonucleotide is capable of reducing expression of a miRNA by at least 50%.