Transgenic plant event detection

1. A method to examine a sample for the presence or absence of material derived from one or more transgenic plant events comprising the steps of:

(1) detecting the presence or absence in the sample of nucleic acids comprising:

one, more than one or all of nucleic acids chosen from:

a) “Zm”: a nucleic acid derived from and specific for Zea mays taxon, preferably Zea mays ssp. mays,

b) “Bn”: a nucleic acid derived from and specific for Brassica napus taxon,

c) “Gm”: a nucleic acid derived from and specific for Glycine max taxon,

o) “Or”: a nucleic acid derived from and specific for Oryza sativa taxon,

p) “Bv”: a nucleic acid derived from and specific for Beta vulgaris taxon,

q) “Gs”: a nucleic acid derived from and specific for Gossypium taxon, and

t) “St”: a nucleic acid derived from and specific for Solanum tuberosum taxon; and

all of nucleic acids d)-i):

d) “p35S”: a nucleic acid derived from the 35S promoter of Cauliflower Mosaic Virus,

e) “tNOS”: a nucleic acid derived from the 3′ terminator of the Agrobacterium tumefaciens nopaline synthetase gene,

f) “Cry1Ab”: a nucleic acid derived from the crystal protein gene Cry1Ab of Bacillus thuringiensis,

g) “PAT/bar”: a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene bar of Streptomyces hygroscopicus,

h) “PAT/pat”: a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene pat of Streptomyces viridochromogenes , and

i) “CP4-EPSPS”: a nucleic acid derived from the 5-Enol-pyruvylshikimate-3-phosphate synthase EPSPS gene from Agrobacterium sp. CP4; and

(2) concluding the presence or absence in the sample of material derived from one or more transgenic plant events chosen from the group comprising: events Bt176, Bt11, Bt10, MON810, MON863, TC1507, NK603, T25, GA21, DAS-59122, MIR604, LY038, MON88017, crosses thereof, and related events thereof; events Topas 19/2, MS1, RF1, RF2, RF3, MS8, GT73, T45, Liberator pHoe6/Ac, GS40/90pHoe6/Ac, OXY235, crosses thereof including MS1/RF1, MS1/RF2,

MS8/RF3, and related events thereof; events MON 40-3-2, MON89788, A2704-12, A5547-127, crosses thereof, and related events thereof, events LL62, LL06 and LL601, crosses thereof, and related events thereof; events T120-7, H7-1 and

A5-15, crosses thereof, and related events thereof; events LL cotton 25, MON 1445, MON 531, MON15985, crosses thereof, and related events thereof; and event EH92-527-1 and related events thereof,

wherein in step (1) the presence or absence of nucleic acids in a sample is detected using PCR amplification, preferably real-time PCR amplification, using the respective primer pairs from the following table:

“Zm”

Fwd: 5′ TCTCTTCCTCCTTTAGAGCTACCACTA 3′

(SEQ ID NO: 56)

Rev: 5′ AATCGATCCAAAGCGAGATGA 3′

(SEQ ID NO: 57)

“Bn”

Fwd: 5′ CAGCTCAACAGTTTCCAAACGA 3′

(SEQ ID NO: 24)

Rev: 5′ CGACCAGCCTCAGCCTTAAG 3′

(SEQ ID NO: 25)

“Gm”

Fwd: 5′ AACCGGTAGCGTTGCCAG 3′

(SEQ ID NO: 58)

Rev′: 5′ AGCCCATCTGCAAGCCTTT 3′

(SEQ ID NO: 59)

“p35S”

Fwd: 5′ AAAGCAAGTGGATTGATGTGATA 3′

(SEQ ID NO: 60)

Rev: 5′ GGGTCTTGCGAAGGATAGTG 3′

(SEQ ID NO: 61)

“tNOS”

Fwd: 5′-GATTAGAGTCCCGCAATTATACATTTAA-3′

(SEQ ID NO: 9)

Rev: 5′-TTATCCTAGKTTGCGCGCTATATTT-3′

(SEQ ID NO: 10)

“Cry1Ab”

Fwd: 5′-ACCGGTTACACTCCCATCGA-3′

(SEQ ID NO: 11)

Rev: 5′-CAGCACCTGGCACGAACTC-3′

(SEQ ID NO: 12)

“PAT/bar”

Fwd: 5′-CGTCAACCACTACATCGAGACAA-3′

(SEQ ID NO: 13)

Rev: 5′-GTCCACTCCTGCGGTTCCT-3′

(SEQ ID NO: 14)

“PAT/pat”

Fwd: 5′-CCGCGGTTTGTGATATCGTT-3′

(SEQ ID NO: 15)

Rev: 5′-TCTTGCXACCTCTCTAGATCATCAA-3′

(SEQ ID NO: 16)

“CP4-EPSPS”

Fwd: 5′ GCATGCTTCACGGTGCAA 3′

(SEQ ID NO: 22)

Rev: 5′ GGACCTGTGGGAGATAGACTTGTC 3′

(SEQ ID NO: 23)

Rev 1: 5′ TGAAGGACCGGTGGGAGAT 3′

(SEQ ID NO: 62)

Rev 2: 5′ TGAAGGACCTGTGGGAGAT 3′

(SEQ ID NO: 63)

“Or”

Fwd′: 5′ GCTTAGGGAACAGGGAAGTAAAGT 3′

(SEQ ID NO: 51)

Rev: 5′ CTTAGCATAGTCTGTGCCATCCA 3′

(SEQ ID NO: 21)

“Bv”

Fwd: 5′ GACCTCCATATTACTGAAAGGAAG 3′

(SEQ ID NO: 64)

Rev: 5′ GAGTAATTGCTCCATCCTGTTCA 3′

(SEQ ID NO: 65)

“Gs”

Fwd: 5′ AGTTTGTAGGTTTTGATGTTACATTGAG 3′

(SEQ ID NO: 66)

Rev: 5′ GCATCTTTGAACCGCCTACTG 3′

(SEQ ID NO: 67)

“St”

Fwd: 5′ GGACATGTGAAGAGACGGAGC 3′

(SEQ ID NO: 68)

Rev: 5′ CCTACCTCTACCCCTCCGC 3′

(SEQ ID NO: 69).

2. The method according to claim 1 , wherein step (1) involves detecting the presence or absence in the sample of nucleic acids comprising all nucleic acids listed under a)-c).

3. The method according to claim 1 ,

wherein step (1) further comprises detecting the presence or absence in the sample of nucleic acid j) “mCry3A”: a nucleic acid derived from the modified crystal protein gene Cry3A of Bacillus thuringiensis ; and/or

wherein step (1) further comprises detecting the presence or absence in the sample of one or both nucleic acids k) “cordapA”: a nucleic acid derived from the lysine-insensitive dihydrodipicolinate synthase (cDHDPS) gene cordapA of Corynebacterium glutamicum and/or I) “Glb1”: a nucleic acid derived from the Glb1 promoter of maize; and/or

wherein step (1) further comprises detecting the presence or absence in the sample of nucleic acid m) “Cry3Bb1”: a nucleic acid derived from the crystal protein gene Cry3Bb1 of Bacillus thuringiensis ; and/or

wherein step (1) further comprises detecting the presence or absence in the sample of nucleic acid n) “Bxn”: a nucleic acid derived from the nitrilase gene Bxn of Klebsiella pneumoniae ssp. ozaenae ; and/or

wherein step (1) further comprises detecting the presence or absence in the sample of one or both of nucleic acids r) “Cry1Ac”: a nucleic acid derived from the crystal protein gene Cry1Ac of Bacillus thuringiensis and/or s) “Cry2Ab2”: a nucleic acid derived from the crystal protein gene Cry2Ab2 of Bacillus thuringiensis ; and/or

wherein step (1) further comprises detecting the presence or absence in the sample of nucleic acid u) “GBSS”: a nucleic acid derived from the granule bound starch synthase gene Gbss of Solanum tuberosum.

4. The method according to claim 1 , wherein step (1) further comprises detecting the presence or absence in the sample of a generic plant-derived nucleic acid, preferably derived from the chloroplastic small subunit of Rubisco gene or from the CHL-tRNA synthetase gene.

5. The method according to claim 4 , wherein the presence or absence of said generic plant-derived nucleic acid is detected using PCR amplification, preferably real-time PCR amplification, using the primer pair 5′ AGGTCTAADGGRTAAGCTAC 3′ (SEQ ID NO: 26) and 5′ AGYCTTGATCGTTACAAAGG 3′ (SEQ ID NO: 27).

6. The method according to claim 1 , wherein the amplification products are detected using a detection method that is substantially sequence non-specific, preferably using a DNA-binding fluorescent dye, more preferably using SYBR Green or PicoGreen.

7. The method according to claim 1 , wherein the specificity of the amplification products is further verified, preferably using melting curve analysis (Tm) and/or size determination by gel elecrophoresis.

8. The method according to claim 1 , wherein the amplification products are detected by means of a fluorophore label present in at least one primer of the primer pair, such as using Light Upon Extension (LUX™) technology.

9. The method according to claim 1 , wherein the sample comprises plants or parts thereof, including flowers, tepals, petals, sepals, anthers, pollen, seeds, fruits, pericarp, pods, leaves, petioles, stems, roots, rhizomes, stolons, tubers or shoots, or portions thereof, plant cells, plant protoplasts and/or plant tissues, and/or plant-derived material, preferably food or feed material, including processed food or feed material.

10. The method according to claim 1 , wherein in step (1) the presence or absence of nucleic acids in a sample is detected using PCR amplification, preferably real-time PCR amplification, using the respective primer pairs from the C following table:

“Zm”

Fwd: 5′ CgTCgTTTCCCATCTCTTCCTCC 3′

(SEQ ID NO: 1)

Rev: 5′ CCACTCCgAgACCCTCAgTC 3′

(SEQ ID NO: 2)

“Zm”

Fwd: 5′ TCTCTTCCTCCTTTAGAGCTACCACTA 3′

(SEQ ID NO: 56)

Rev: 5′ AATCGATCCAAAGCGAGATGA 3′

(SEQ ID NO: 57)

“Bn”

Fwd1l: 5′ GAGAATGAGGAGGACCAAGCTC 3′

(SEQ ID NO: 4)

Fwd2: 5′GGTGAGCTGTATAATCGAGCGA 3′

(SEQ ID NO: 79)

Rev: 5′ GGCGCAGCATCGGCT 3′

(SEQ ID NO: 3)

“Bn”

Fwd: 5′ CAGCTCAACAGTTTCCAAACGA 3′

(SEQ ID NO: 24)

Rev: 5′ CGACCAGCCTCAGCCTTAAG 3′

(SEQ ID NO: 25)

“Gm”

Fwd: 5′ CATTACCTATgATgCCTCCACC 3′

(SEQ ID NO: 5)

Rev: 5′ AAgCACgTCATgCgATTC 3′

(SEQ ID NO: 6)

Rev′: 5′ AAgCACgTCATgCgATTCC 3′

(SEQ ID NO: 49)

“Gm”

Fwd: 5′ AACCGGTAGCGTTGCCAG 3′

(SEQ ID NO: 58)

Rev′: 5′ AGCCCATCTGCAAGCCTTT 3′

(SEQ ID NO: 59)

“p35S”

Fwd: 5′-GACAGTGGTCCCAAAGATGG-3′

(SEQ ID NO: 7)

Rev: 5′-GTCTTGCGAAGGATAGTGGG-3′

(SEQ ID NO: 8)

“p35S”

Fwd: 5′ AAAGCAAGTGGATTGATGTGATA 3′

(SEQ ID NO: 60)

Rev: 5′ GGGTCTTGCGAAGGATAGTG 3′

(SEQ ID NO: 61)

“tNOS”

Fwd: 5′-GATTAGAGTCCCGCAATTATACATTTAA-3′

(SEQ ID NO: 9)

Rev: 5′-TTATCCTAGKTTGCGCGCTATATTT-3′

(SEQ ID NO: 10)

“tNOS”

Fwd: 5′ CGTTCAAACATTTGGCAATAAAG 3′

(SEQ ID NO: 28)

Rev: 5′ AAATGTATAATTGCGGGACTCTAATC 3′

(SEQ ID NO: 29)

“Cry1Ab”

Fwd: 5′-ACCGGTTACACTCCCATCGA-3′

(SEQ ID NO: 11)

Rev: 5′-CAGCACCTGGCACGAACTC-3′

(SEQ ID NO: 12)

“Cry1Ab”

Fwd: 5′ GACATCATCTGGGGYATCTT 3′

(SEQ ID NO: 30)

Rev: 5′ GCGCTGTTCATGTCGTTGAA 3′

(SEQ ID NO: 31)

“Cry1Ab”

Fwd: 5′ ACGCCTTCCTGGTGCAAA 3′

(SEQ ID NO: 47)

Rev: 5′ CCTGGTTCCTGGCGAACTC 3′

(SEQ ID NO: 48)

“PAT/bar”

Fwd: 5′-CGTCAACCACTACATCGAGACAA-3′

(SEQ ID NO: 13)

Rev: 5′-GTCCACTCCTGCGGTTCCT-3′

(SEQ ID NO: 14)

“PAT/pat”

Fwd: 5′-CCGCGGTTTGTGATATCGTT-3′

(SEQ ID NO: 15)

Rev: 5′-TCTTGCAACCTCTCTAGATCATCAA-3′

(SEQ ID NO: 16)

“CP4-EPSPS”

Fwd1: 5′-GGCTCTGAGCTTCGTCCTCCTAAGG-3′

(SEQ ID NO: 17)

Fwd1′: 5′-GGCTCTGAGCTTCGTCCTCTTAAGG-3′

(SEQ ID NO: 50)

Fwd2: 5′-ATCAGTGGCTACAGCCTGCAT-3′

(SEQ ID NO: 18)

Rev: 5′-GAATGCGGACGGTTCCGGAAAG-3′

(SEQ ID NO: 19)

“CP4-EPSPS”

Fwd: 5′ GCATGCTTCACGGTGCAA 3′

(SEQ ID NO: 22)

Rev: 5′ GGACCTGTGGGAGATAGACTTGTC 3′

(SEQ ID NO: 23)

Rev 1: 5′ TGAAGGACCGGTGGGAGAT 3′

(SEQ ID NO: 62)

Rev 2: 5′ TGAAGGACCTGTGGGAGAT 3′

(SEQ ID NO: 63)

“Or”

Fwd: 5′ GCTTAGGGAACAGGGAAGTAAAGTT 3′

(SEQ ID NO: 20)

Fwd′: 5′ GCTTAGGGAACAGGGAAGTAAAGT 3′

(SEQ ID NO: 51)

Rev: 5′ CTTAGCATAGTCTGTGCCATCCA 3′

SEQ ID NO: 21

“Bv”

Fwd: 5′ GACCTCCATATTACTGAAAGGAAG 3′

(SEQ ID NO: 64)

Rev: 5′ GAGTAATTGCTCCATCCTGTTCA 3′

(SEQ ID NO: 65)

“Gs”

Fwd: 5′ AGTTTGTAGGTTTTGATGTTACATTGAG 3′

(SEQ ID NO: 66)

Rev: 5′ GCATCTTTGAACCGCCTACTG 3′

(SEQ ID NO: 67)

“St”

Fwd: 5′ GGACATGTGAAGAGACGGAGC 3′

(SEQ ID NO: 68)

Rev: 5′ CCTACCTCTACCCCTCCGC 3′

(SEQ ID NO: 69)

generic plant

Fwd: 5′ AGGTCTAADGGRTAAGCTAC 3′

(SEQ ID NO: 26)

Rev: 5′ AGYCTTGATCGTTACAAAGG 3′

(SEQ ID NO: 27)

11. The method according to claim 1 , wherein the step (2) comprises the steps of:

(i) defining a set “G SAM ” consisting of nucleic acids detected in step (1) as being present in the sample;

(ii) defining one or more set of interest (“G X ”) chosen from the group of sets comprising or consisting of sets “G Bt176 ”, “G Bt11 ”, “G Bt10 ”, “G MON810 ”, “G MON863 ”, “G TC1507 ”, “G NK603 ”, “G T25 ”, “G GA21 ”, “G DAS-59122 ”, “G MIR604 ”, “G LY038 ”, “G MON88017 ”, “G Topas 19/2 ”, “G MS1 ”, “G RF1 ”, “G RF2 ”, “G MS1/RF1 ”, “G MS1/RF2 ”, “G MS8 ”, “G RF3 ”, “G MS8/RF3 ”, “G GT73 ”, “G T45 ”, “G Liberator pHoe6/Ac ”, “G GS40/90pHoe6/Ac ”, “G OXY235 ”; “G MON40-3-2 ”, “G MON89788 ”, “G A2704-12 ”, “G A5547-127 ”, “G LL62 ”, “G LL06 ”, “G LL601 ”, “G T120-7 ”, “G H7-1 ”, “G A5-15 ”, “G LL cotton 25 ”, “G MON1445 ”, “G MON531 ”, “G MON15985 ” and “G EH92-527-1 ”; corresponding to the respective one or more transgenic plant events of interest (“X”) chosen from the group comprising or consisting of events Bt176, Bt11, Bt10, MON810, MON863, TC1507, NK603, T25, A21, DAS-59122, MIR604, LY038, MON88017, Topas 19/2, MS1, RF1, RF2, MS1/RF1, MS1/RF2, MS8, RF3, MS8/RF3, G T73, T45, Liberator pHoe6/Ac, GS40/90pHoe6/Ac, OXY235; MON40-3-2, MON89788, A2704-12, A5547-127, LL62, LL06, LL601, T120-7, H7-1, A5-15, LL cotton 25, MON1445, MON531, MON15985 and EH92-527-1, wherein:

G Bt176 ε{Zm; p35S; Cry1Ab; PAT/bar},

G Bt11 ε{Zm; p35S; tNOS; Cry1Ab; PAT/pat},

G Bt10 ε{Zm; p35S; tNOS; Cry1Ab; PAT/pat},

G MON810 ε{Zm; p35S; tNOS; Cry1Ab},

G MON863 ε{Zm; p35S; tNOS},

G TC1507 ε{Zm; p35S; PAT/pat},

G NK603 ε{Zm; p35S; tNOS; CP4-EPSPS},

G T25 ε{Zm; p35S; PAT/pat},

G GA21 ε{Zm; tNOS},

G DAS-59122 ε{Zm; p35S; PAT/bar},

G MIR604 ε{Zm; tNOS; mCry3A}, or G MIR604 ε{Zm; tNOS}

G LY038 ε{Zm; p35S; tNOS; cordapA; Glb1}, or G LY038 ε{Zm; p35S; tNOS},

G MON88017 ε{Zm; p35S; tNOS; CP4-EPSPS; Cry3Bb1}, or G MON88017 ε{Zm; p35S; tNOS; CP4-EPSPS},

G Topas 19/2 ε{Bn; p35S; PAT/pat},

G MS1 ε{Bn; tNOS; PAT/bar},

G RF1 ε{Bn; tNOS; PAT/bar},

G RF2 ε{Bn; tNOS; PAT/bar},

G MS1/RF1 ε{Bn; tNOS; PAT/bar},

G MS1/RF2 ε{Bn; tNOS; PAT/bar},

G MS8 ε{Bn; tNOS; PAT/bar},

G RF3 ε{Bn; tNOS; PAT/bar},

G MS8/RF3 ε{Bn; tNOS; PAT/bar},

G GT73 ε{Bn; CP4-EPSPS},

G T45 ε{Bn; p35S; PAT/pat},

G Liberator pHoe6/Ac ε{Bn; p35S; PAT/pat},

G GS40/90pHoe6/Ac ε{Bn; p35S; PAT/pat},

G OXY235 ε{Bn; p35S; Bxn}, or G OXY235 ε{Bn; p35S};

G MON40-3-2 ε{Gm; p35S; tNOS; CP4-EPSPS},

G MON89788 ε{Gm; CP4-EPSPS}

G A2704-12 ε{Gm; p35S; PAT/pat},

G A5547-127 ε{Gm; p35S; PAT/pat},

G LL62 ε{Or; p35S; PAT/bar},

G LL06 ε{Or; p35S; PAT/bar}

G LL601 ε{Or; p35S; tNOS; PAT/bar},

G T120-7 ε{Bv; p35S; PAT/pat},

G H7-1 ε{Bv; p35S; CP4-EPSPS},

G A5-15 ε{Bv; p35S; tNOS; CP4-EPSPS},

G LL cotton 25 ε{Gs; p35S; tNOS; PAT/bar},

G MON1445 ε{Gs; p35S; tNOS; CP4-EPSPS},

G MON531 ε{Gs; p35S; tNOS; cry1Ac}, or G MON531 ε{Gs; p35S; tNOS}

G MON15985 ε{Gs; p35S; tNOS; cry1Ac; cry2Ab2} or G MON15985 ε{Gs; p35S; tNOS}, and

G EH92-527-1 ε{St; tNOS; Gbss} or G EH92-527-1 ε{St; tNOS};

(iii) performing for each set of interest G X logical operations:

if G X equals G SAM (G X =G SAM ), then material derived from the transgenic plant event X or from a cross thereof, or from an event related thereto, is potentially present in the sample;

if G X is a proper subset of G SAM (G X ⊂G SAM ), then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is potentially present in the sample;

if G X does not equal G SAM and G X is not a proper subset of G SAM , (G X ≠G SAM and G X ⊂G SAM ), then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is absent from the sample.

12. The method according to claim 11 , wherein if G X equals G SAM , and if no one set or the sum of two or more sets chosen from the group comprising or consisting of G Bt176 , G Bt11 , G Bt10 , G MON810 , G MON863 , G TC1507 , G NK603 , G T25 , G GA21 , G DAS-59122 , G MIR604 , G LY038 , G MON88017 , G Topas 19/2 , G MS1 , G RF1 , G RF2 , G MS1/RF1 , G MS1/RF2 , G MS8 , G RF3 , G MS8/RF3 , G GT73 , G T45 , G Liberator pHoe6/Ac , G GS40/90pHoe6/Ac , G OXY235 ; G MON40-3-2 , G MON89788 , G A2704-12 , G A5547-127 , G LL62 , G LL06 , G LL601 , G T120-7 , G H7-1 , G A5-15 , G LL cotton 25 , G MON1445 , G MON531 , G MON15985 other than G X equals G X , then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is present in the sample.

13. The method according to claim 11 , wherein nucleic acids chosen from the group comprising or consisting of “Zm”, “Bn”, “Gm”, “p35S”, “tNOS”, “Cry1Ab”, “PAT/bar”, “PAT/pat”, “CP4-EPSPS”, “mCry3A”, “cordap A”, “Glb1”, “Cry3Bb1”, “Bxn”, “Or”, “Bv”, “Gs”, “Cry1Ac”, “Cry2ab2”, “St” and “Gbss” are assigned respective unique values “V Zm ”, “V Bn ”, “V Gm ”, “V p35S ”, “V tNOS ”, “V Cry1Ab ”, “V PAT/bar ”, “V PAT/pat ”, “V CP4-EPSPS ”, “V mCry3A ”, “V cordap A ”, “V Glb1 ”, “V Cry3Bb1 ”, “V Bxn ”, “V Or ”, “V Bv ”, “V Gs ”, “V Cry1Ac ”, “V Cry2ab2 ”, “V St ” and “V Gbss ”; wherein each set of interest G X is assigned a value “VG X ” chosen from the group comprising consisting of values “VG Bt176 ”, “VG Bt11 ”, “VG Bt10 ”, “VG MON810 ”, “VG MON863 ”, “VG TC1507 ”, “VG NK603 ”, “V”G T25 ”, “VG GA21 ”, “VG DAS-59122 ”, “VG MIR604 ”, “VG LY038 ”, “VG MON88017 ”, “VG Topas 19/2 ”, “VG MS1 ”, “VG RF1 ”, “VG RF2 ”, “VG MS1/RF1 ”, “VG MS1/RF2 ”, “VG MS8 ”, “VG RF3 ”, “VG MS8/RF3 ”, “VG GT73 ”, “VG T45 ”, “VG Liberator pHoe6/Ac ”, “VG GS40/90pHoe6/Ac ”, “VG OXY235 ”, “VG MON40-3-2 ”, “VG MON89788 ”, “VG A2704-12 ”, “VG A5547-127 ”, “VG LL62 ”, “VG LL06 ”, “VG LL601 ”, “VG T120-7 ”, “VG H7-1 ”, “VG A5-15 ”, “VG LL cotton 25 ”, “VG MON1445 ”, “VG MON531 ”, “VG MON15985 ” and “VG EH92-527-1 ”, wherein:

VG Bt176 =V Zm ×V p35S ×V Cry1Ab ×V PAT/bar ,

VG Bt11 =V Zm ×V p35S ×V tNOS ×V Cry1Ab ×V PAT/pat ,

VG Bt10 =V Zm ×V p35S ×V tNOS ×V Cry1Ab ×V PAT/pat ,

VG MON810 =V Zm ×V p35S ×V tNOS ×V Cry1Ab ,

VG MON863 =V Zm ×V p355 ×V tNOS ,

VG TC1507 =V Zm ×V p35S ×V PAT/pat ,

VG NK603 =V Zm ×V p35S ×V tNOS ×V CP4-EPSPS ,

VG T25 =V Zm ×V p35S ×V PAT/pat ,

VG GA21 =V Zm ×V tNOS ,

VG DAS-59122 =V Zm ×V p35S ×V PAT/bar ,

VG MIR604 =V Zm ×V tNOS ×V mCry3A , or VG MIR604 =V Zm ×V tNOS ,

VG LY038 =V Zm ×V p35S ×V tNOS ×V cordapA ×V Glb1 , or VG LY038 =V Zm ×V p35S ×V tNOS ,

VG MON88017 =V Zm ×V p35S ×V tNOS ×V CP4-EPSP ×V Cry3Bb1 , or VG MON88017 =V Zm ×V p35S ×V tNOS ×V CP4-EPSP ,

VG Topas 19/2 =V Bn ×V p35S ×V PAT/pat ,

VG MS1 =V Bn ×V tNOS ×V PAT/bar ,

VG RF1 =V Bn ×V tNOS ×V PAT/bar ,

VG RF2 =V Bn ×V tNOS ×V PAT/bar ,

VG MS8 =V Bn ×V tNOS ×V PAT/bar ,

VG RF3 =V Bn ×V tNOS ×V PAT/bar ,

VG MS1/RF1 =V Bn ×V tNOS ×V PAT/bar ,

VG MS1/RF2 =V Bn ×V tNOS ×V PAT/bar ,

VG MS8/RF3 =V Bn ×V tNOS ×V PAT/bar ,

VG GT73 =V Bn ×V CP4-EPSPS ,

VG T45 =V Bn ×V p35S ×V PAT/pat ,

VG Liberator pHoe6/Ac =V Bn ×V p35S ×V PAT/pat ,

VG GS40/90pHoe6/Ac =V Bn ×V p35S ×V PAT/pat ,

VG OXY235 =V Bn ×V p35S ×V Bxn , or VG OXY235 =V Bn ×V p35S ,

VG MON40-3-2 =V Gm ×V p35S ×V tNOS ×V CP4-EPSPS ,

VG MON89788 =V Gm ×V CP4-EPSPS ,

VG A2704-12 =V Gm ×V p35S ×V PAT/pat ,

VG A5547-127 =V Gm ×V p35S ×V PAT/pat ,

VG LL62 =V Or ×V p35S ×V PAT/bar ,

VG LL06 =V Or ×V p35S ×V PAT/bar ,

VG LL601 =V Or ×V p35S ×V tNOS ×V PAT/bar ,

VG T120-7 =V Bv ×V p35S ×V PAT/pat ,

VG H7-1 =V Bv ×V p35S ×V CP4-EPSPS ,

VG A5-15 =V Bv ×V p35S ×V tNOS ×V CP4-EPSPS ,

VG LL cotton 25 =V Gs ×V p35S ×V tNOS ×V PAT/bar ,

VG MON1445 =V Gs ×V p35S ×V tNOS ×V CP4-EPSPS ,

VG MON531 =V Gs ×V p35S ×V tNOS ×V Cry1Ac , or VG MON531 =V Gs ×V p35S ×V tNOS ,

VG MON15985 =V Gs ×V p35S ×V tNOS ×V Cry1Ac ×V Cry2Ab2 , or VG MON15985 =V Gs ×V p35S ×V tNOS ,

and VG EH92-527-1 V St ×V tNOS ×V Gbss , or VG EH92-527-1 =V St ×V tNOS ,

and wherein the set G SAM is assigned a value “VG SAM ” which is a multiple of the unique values assigned to the nucleic acids detected in step (1) as being present in the sample;

and wherein step (iii) comprises performing for each set of interest G X logical operations:

if VG SAM /VG X equals 1, then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is potentially present in the sample;

if VG SAM /VG X equals one or a multiple of two or more values chosen from a group comprising or consisting of values V Zm , V Bn , V Gm , V p35S , V tNOS , V Cry1Ab , V PAT/bar , V PAT/pat , V CP4-EPSPS , V mCry3A , V cordap A , V Glb1 , V Cry3Bb1 , V Bxn , V Or , V Bv , V Gs , V Cry1Ac , V Cry2ab2 , V St and V Gbss , then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is potentially present in the sample;

if VG SAM /VG X does not equal 1 and does not equal one value or a multiple of two or more values chosen from a group comprising or consisting of values V Zm , V Bn , V Gm , V p35S , V tNOS , V Cry1Ab , V PAT/bar , V PAT/pat , V CP4-EPSPS , V mCry3A , V cordap A , V Glb1 , V Cry3Bb1 , V Bxn , V Or , V Bv , V Gs , V Cry1Ac , V Cry2ab2 , V St and V Gbss , then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is absent from the sample.

14. The method according to claim 13 , wherein if VG SAM /VG X equals 1, and if no one value or the multiple of two or more values of sets chosen from a group comprising or consisting of: VG Bt176 , VG Bt11 , VG Bt10 , VG MON810 , VG MON863 , VG TC1507 , VG NK603 , VG T25 , VG GA21 , VG DAS-59122 , VG MIR604 , VG LY038 , VG MON88017 , VG Topas 19/2 , VG MS1 , VG RF1 , VG RF2 , VG MS1/RF1 , VG MS1/RF2 , VG MS8 , VG RF3 , VG MS8/RF3 , VG GT73 , VG T45 , VG Liberator pHoe6/Ac , VG GS40/90pHoe6/Ac , VG OXY235 , VG MON40-3-2 , VG MON89788 , VG A2704-12 , VG A5547-127 , VG LL62 , VG LL06 , VG LL601 , VG T120-7 , VG H7-1 , VG A5-15 , VG LL cotton 25 , VG MON1445 , VG MON531 , VG MON15985 and VG EH92-527-1 other than VG X equals VG X , then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is present in the sample.

15. The method according claim 13 , wherein values V Zm , V Bn , V Gm , V p35S , V tNOS , V Cry1Ab , V PAT/bar , V PAT/pat , V CP4-EPSPS , V mCry3A , V cordap A , V Glb1 , V Cry3Bb1 , V Bxn , V Or , V Bv , V Gs , V Cry1Ac , V Cry2ab2 , V St and V Gbss are unique prime numbers, and wherein step (iii) comprises performing for each set of interest G X logical operations,

if VG SAM /VG X equals 1, then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is potentially present in the sample;

if VG SAM /VG X is an integer greater than 1, then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is potentially present in the sample;

if VG SAM /VG X is not an integer, then material derived from transgenic plant event X or from a cross thereof, or from an event related thereto, is absent from the sample.

16. The method according to claim 1 , wherein step (2) is carried out by a computing device.

17. The method according to claim 1 , wherein the PCR amplification of the nucleic acids is performed simultaneously at the same temperature cycling conditions.

18. The method according to claim 1 , wherein the PCR amplification of two or more of the nucleic acids is multiplexed.

19. A computing device programmed to carry out step (2) of the method according to claim 1 ; or a data carrier, that is not a carrier wave, comprising instructions for a programmable computing device to carry out the step (2) of the method according to claim 1 .