Transgenic plants containing soluble cell wall polysaccharides
The present invention provides a bio-fuel, wood or other product, such as a paper, textile or yarn product. The product can contain material from a transgenic plant over-expressing a nucleic acid molecule encoding an enzyme that causes the plant cell wall to be more water soluble than the wild type.
1. A transgenic plant expressing a nucleic acid sequence encoding a cellobiose dehydrogenase (CDH) polypeptide, wherein said polypeptide is expressed in the cell wall of the plant using a nucleic acid sequence encoding a signal peptide, wherein the cell wall of the transgenic plant is more water soluble than the cell wall of the wild type, and said CDH is obtained from P. chrysosporium.
2. The transgenic plant of claim 1 , wherein said plant is selected from the group consisting of a monocotyledonous and dicotyledonous plant, grain crop, forage crop, root vegetable crop, woody plant, conifer, pine tree, poplar, willow, eucalyptus, acacia, oil palm, sugar cane, Jerusalem artichoke and perennial grass.
3. The transgenic plant of claim 2 , wherein said root vegetable crop is selected from the group consisting of carrot, potato, sugar beets and yam.
4. The transgenic plant of claim 2 , wherein said pine tree is selected from the group consisting of pine fir, loblolly pine, radiata pine and spruce.
5. The transgenic plant of claim 2 , wherein said perennial grass is switch grass or miscanthus.
6. The transgenic plant of claim 1 , wherein the cellulose in the cell wall of the transgenic plant is less crystalline than the cellulose in the cell wall of the wild type.
7. The transgenic plant of claim 1 , wherein the lignin content in the cell wall of the transgenic plant is less than the lignin content in the cell wall of the wild type.
8. The transgenic plant of claim 1 , wherein the transgenic plant is more amenable than the wild type for processing into a bio-fuel, wood, paper, textile or yarn product.
9. A method of making a bio-fuel, wood, paper, textile or yarn product, comprising: a) expressing a nucleic acid molecule encoding CDH in a plant, said CDH including a signal peptide sequence that can send said CDH to the plant cell wall; and b) processing the cellulose or hemicellulose portion of the plant into said product, wherein the cell wall of the transgenic plant is more water soluble than the cell wall of the wild type, and said CDH is obtained from P. chrysosporium.
10. The method of claim 9 , wherein the product is a bio-fuel.
11. The method of claim 10 , wherein said bio-fuel is ethanol or butanol.
12. The method of claim 11 , wherein said bio-fuel is ethanol.
13. The transgenic plant of claim 1 , wherein said plant is a woody plant.
14. The method of claim 9 , wherein said plant is a woody plant.
15. A transgenic plant expressing a nucleic acid sequence encoding a cellobiose dehydrogenase (CDH) polypeptide, wherein said polypeptide is expressed in the cell wall of the plant using a nucleic acid sequence encoding a signal peptide, wherein the cell wall of the transgenic plant is more water soluble than the cell wall of the wild type and said CDH comprises SEQ ID NO: 6.
16. The transgenic plant of claim 15 , wherein said plant is selected from the group consisting of a monocotyledonous and dicotyledonous plant, grain crop, forage crop, root vegetable crop, woody plant, conifer, pine tree, poplar, willow, eucalyptus , acacia, oil palm, sugar cane, Jerusalem artichoke and perennial grass.
17. The transgenic plant of claim 15 , wherein the transgenic plant is more amenable than the wild type for processing into a bio-fuel, wood, paper, textile or yarn product.
18. A method of making a bio-fuel, wood, paper, textile or yarn product, comprising: a) expressing a nucleic acid molecule encoding CDH in a plant, said CDH including a signal peptide sequence that can send said CDH to the plant cell wall; and b) processing the cellulose or hemicellulose portion of the plant into said product, wherein the cell wall of the transgenic plant is more water soluble than the cell wall of the wild type and said CDH comprises SEQ ID NO: 6.
19. The method of claim 10 , wherein said bio-fuel is ethanol or butanol.