Spatial light interference microscopy and fourier transform light scattering for cell and tissue characterization
View Patent ↗Methods and apparatus for rendering quantitative phase maps across and through transparent samples. A broadband source is employed in conjunction with an objective, Fourier optics, and a programmable two-dimensional phase modulator to obtain amplitude and phase information in an image plane. Methods, referred to as Fourier transform light scattering (FTLS), measure the angular scattering spectrum of the sample. FTLS combines optical microscopy and light scattering for studying inhomogeneous and dynamic media. FTLS relies on quantifying the optical phase and amplitude associated with a coherent image field and propagating it numerically to the scattering plane. Full angular information, limited only by the microscope objective, is obtained from extremely weak scatterers, such as a single micron-sized particle. A flow cytometer may employ FTLS sorting.
1. A method for deriving a quantitative phase contrast image of a specimen, the method comprising steps of:
a. illuminating the specimen through a condenser annulus that introduces a phase delay that is an integral multiple of π/2 with illuminating light characterized by a coherence length substantially shorter than 10 micrometers;
b. scanning a focus to a plurality of depths within the specimen;
c. spatially Fourier transforming light transmitted through the specimen at each of the plurality of depths;
d. temporally modulating at least one of the phase and amplitude of the spatial frequency components of the spatially transformed image to obtain interferograms at a plurality of relative phase offsets;
e. detecting an intensity image of the specimen plane as modulated with respect to spatial frequency components; and
f. deconvolving the intensity image at a plurality of spatially modulated instances to obtain a three-dimensional phase representation of the specimen at each of a plurality of distances relative to a fiducial plane.
2. A method in accordance with claim 1 , wherein the illuminating light is characterized by a coherence length no greater than about 1.5 microns.
3. A method in accordance with claim 1 , wherein the illuminating light is focused onto the specimen with an optic characterized by a numerical aperture exceeding 0.5.
4. A method in accordance with claim 1 , wherein the specimen is a substantially cylindrical object.
5. A method in accordance with claim 4 , wherein the specimen is a nanotube.
6. A method in accordance with claim 1 , further comprising identifying a central frequency with respect to which phase differences are defined.