Recombinant polyprotein vaccines for the treatment and diagnosis of leishmaniasis
Compositions and methods for preventing, treating and detecting leishmaniasis are disclosed. The compositions generally comprise fusion polypeptides comprising multiple Leishmania antigens, in particular, KMP11, SMT, A2 and/or CBP, or immunogenic portions or variants thereof, as well as polynucleotides encoding such fusion polypeptides.
1. A fusion polypeptide comprising the amino acid sequence set forth as SEQ ID NO:33, or a sequence having at least 90% identity to SEQ ID NO:33.
2. The fusion polypeptide of claim 1 , wherein the fusion polypeptide comprises the amino acid sequence set forth as SEQ ID NO:33.
3. The fusion polypeptide of claim 1 , wherein the fusion polypeptide comprises a sequence having at least 95% identity to SEQ ID NO: 33.
4. A composition comprising a fusion polypeptide comprising the amino acid sequence set forth as SEQ ID NO:33 or a sequence haying at least 90% identity to SEQ ID NO:33 and at least one immunostimulant.
5. The composition according to claim 4 , wherein the immunostimulant is selected from the group consisting of a CpG-containing oligonucleotide, synthetic lipid A, monophosphoryl lipid A, 3-de-O-acylated monophosphoryl lipid A, saponins, saponin mimetics, AGPs, Toll-like receptor agonists, or a combination thereof.
6. The composition according to claim 4 , wherein the immunostimulant is selected from the group consisting of a TLR4 agonist, a TLR7/8 agonist and a TLR9 agonist.
7. The composition according to claim 4 , wherein the immunostimulant is selected from the group consisting of GLA, CpG-containing oligonucleotide, imiquimod, gardiquimod and resiquimod.
8. The composition of claim 4 , wherein the fusion polypeptide comprises the amino acid sequence set forth as SEQ ID NO: 33.
9. The composition of claim 4 , wherein the fusion polypeptide comprises a sequence having at least 95% identity to SEQ ID NO: 33.
10. A diagnostic kit for detecting Leishmania infection in a biological sample comprising: (a) a fusion polypeptide comprising the amino acid sequence set forth as SEQ ID NO:33 or a sequence haying at least 90% identity to SEQ ID NO:33; and (b) a detection reagent.
11. The diagnostic kit of claim 10 , wherein the fusion polypeptide comprises the amino acid sequence set forth as SEQ ID NO: 33.
12. The diagnostic kit of claim 10 , wherein the fusion polypeptide comprises a sequence having at least 95% identity to SEQ ID NO: 33.