IP Library › Granted Patent US 8,283,177
Granted Patent B2
US 8,283,177 · App. 12/476,860 · Granted Oct 9, 2012

Fluidic system with washing capabilities for a flow cytometer

Assignee: Accuri Cytometers, Inc.
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Quick Facts
Patent No.
US 8,283,177
App. No.
12/476,860
Granted
Oct 9, 2012
Kind
B2
Abstract

A method for cleaning a fluidic system of a flow cytometer having a sheath pump to pump sheath fluid towards an interrogation zone and a waste pump to pump the sheath fluid and a sample fluid as waste fluid from the interrogation zone, wherein the sheath pump and/or the waste pump draw sample fluid into the flow cytometer through a drawtube towards the interrogation zone. The method includes controlling the sheath pump and the waste pump to cooperatively flush a fluid out through the drawtube, thereby cleaning the fluidic system of the flow cytometer.

Claims (19)

1. A method for cleaning a fluidic system of a flow cytometer having a sheath pump to pump sheath fluid towards an interrogation zone and a waste pump to pump the sheath fluid and a sample fluid as waste fluid from the interrogation zone, wherein the sheath pump and/or the waste pump draw sample fluid into the flow cytometer through a drawtube towards the interrogation zone, comprising:

controlling the sheath pump and the waste pump to cooperatively flush a fluid out through the drawtube, thereby cleaning the fluidic system of the flow cytometer, including: pumping sheath fluid towards the interrogation zone of the flow cytometer at a first flow rate; and pumping waste fluid from the interrogation zone at a second flow rate; wherein the first flow rate is greater than the second flow rate; and

drawing in a cleaning fluid, including: pumping sheath fluid towards the interrogation zone of the flow cytometer at a third flow rate; and pumping waste fluid from the interrogation zone at a fourth pump flow rate; wherein the third flow rate is less than the fourth flow rate.

2. The method of claim 1 , further including depositing flushed out fluid into a wash station.

3. The method of claim 2 , further including inserting the drawtube into the wash station.

4. The method of claim 3 , further including wiping the outside of the drawtube with a drawtube scrubber.

5. The method of claim 4 , wherein the drawtube scrubber is a film that abrasively rubs against the drawtube as the drawtube is inserted into the wash station.

6. The method of claim 3 , further including rinsing the outside of the drawtube in fluid collected in a convex cavity of the wash station.

7. The method of claim 6 , further including pumping fluid through a channel created by the outside of the drawtube and the walls of the cavity of the wash station.

8. The method of claim 1 , wherein the first flow rate and the third flow rate are different flow rates.

9. The method of claim 1 , wherein the second flow rate is approximately zero.

10. The method of claim 1 , wherein drawing in a cleaning fluid includes:

drawing in a cleaning fluid that comprises at least one of sheath fluid flushed out of the drawtube and a cleaning agent in a wash station.

11. A method for cleaning a fluidic system of a flow cytometer having a sheath pump to pump sheath fluid towards an interrogation zone and a waste pump to pump the sheath fluid and a sample fluid as waste fluid from the interrogation zone, wherein the sheath pump and/or the waste pump draw sample fluid into the flow cytometer through a drawtube towards the interrogation zone, comprising:

controlling the sheath pump and the waste pump to cooperatively flush a fluid out of the fluidic system through the drawtube, thereby cleaning the fluidic system of the flow cytometer, including: pumping sheath fluid towards the interrogation zone of the flow cytometer at a first flow rate and pumping waste fluid from the interrogation zone at a second flow rate, wherein the first flow rate is greater than the second flow rate; and

drawing in a cleaning fluid that is a mixture of sheath fluid flushed out of the drawtube and a cleaning agent in a wash station, wherein drawing in a cleaning fluid includes: pumping sheath fluid towards the interrogation zone of the flow cytometer at a third flow rate; and pumping waste fluid from the interrogation zone at a fourth pump flow rate; wherein the third flow rate is less than the fourth flow rate.

12. The method of claim 11 , further comprising inserting the drawtube into the wash station and depositing flushed out fluid into a wash station.

13. The method of claim 11 , wherein the first flow rate and the third flow rate are different flow rates.

14. The method of claim 11 , wherein the second flow rate is approximately zero.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 4, 2010
From: BALL, JACK T.; KEHRER, AARON; BAIR, NATHANIEL C.; RICH, COLLIN A.
To: ACCURI CYTOMETERS, INC.
Reel/Frame 024789/0020 →
Continuity (4)
Continuation In Part 11370714 · Mar 8, 2006
Provisional Application 61082035 · Jul 18, 2008
Provisional Application 61088660 · Aug 13, 2008
Related Publication 20090293910A1 · Dec 3, 2009