Biochip and apparatus for detecting biomaterial using biochip
View Patent ↗Provided is a biochip and an apparatus for detecting a biomaterial. The biochip includes a metal thin film on the surface of a substrate, restraining autofluorescence of the substrate, and a spacer on the metal thin film, having capture molecules immobilized on the surface of the spacer and specifically bound to target molecules. The spacer has a thickness controlled to enhance the strength of a fluorescence signal emitted from a fluorophore labeled with the target molecules and immobilized on the spacer by the specific binding between the capture molecule and the target molecule.
1. A biochip comprising:
a metal thin film on the surface of a substrate, configured to restrain autofluorescence of the substrate;
a spacer disposed over the thin metal film and having a predetermined thickness;
a plurality of capture molecules immobilized on the surface of the spacer;
a first portion of the plurality of capture molecules that are specifically bound to target molecules;
a second portion of the plurality of capture molecules that are non-specifically bound to fluorescent detection molecules;
a first fluorophore layer comprising a plurality of fluorescent detection molecules coupled, by binding to the target molecule that are specifically bound, to the first portion of the plurality of capture molecules; and
a second fluorophore layer comprising a plurality of fluorescent detection molecules non-specifically bound to the second portion of the plurality of capture molecules;
wherein the predetermined thickness is of a thickness determined to cause constructive interference between light directly emitted from the first fluorophore layer and light emitted from the first fluorophore layer and reflected by the metal thin film, and to restrain light emitted from the second fluorophore layer.
2. The biochip of claim 1 , wherein the substrate is formed of any selected from the group consisting of polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polycarbonate (PC), polyamide (PA), polyethylene (PE), polypropylene (PP), polyphenylene ether (PPE), polystyrene (PS), polyoxymethylene (POM), polyetheretherketone (PEEK), polytetrafluoroethylene (PTFE), polyvinylchloride (PVC), polyvinylidene fluoride (PVDF), polybutyleneterephthalate (PBT), fluorinated ethylenepropylene (FEP), and perfluoralkoxyalkane (PFA).
3. The biochip of claim 1 , wherein the metal thin film is formed of any selected from the group consisting of Ag, Cr, Ni, Al, and Ti.
4. The biochip of claim 1 , wherein the spacer is formed of any selected from the group consisting of an oxide, a nitride, and an inorganic material.
5. The biochip of claim 1 , wherein the spacer further comprises a polymer selected from the group consisting of a poly lysine and a self-assembled molecular monolayer (SAM).
6. The biochip of claim 1 , wherein the capture molecules are immobilized by any selected from the group consisting of thiol (—SH), hydroxyl (—OH), silane, amine and epoxy, which are induced on the surface of the spacer.
7. The biochip of claim 1 , wherein the fluorophores of the first and second fluorophore layers have a beaded shape comprising a plurality of fluorescent particles.
8. The biochip of claim 1 , wherein the fluorophore is selected from the group consisting of a platinum, a strontium aluminate, and a zinc sulfide.
9. An apparatus for detecting a biomaterial, the apparatus comprising:
a metal thin film on the surface of a substrate, restraining autofluorescence of the substrate;
a spacer disposed over the thin metal film and having a predetermined thickness;
a plurality of capture molecules immobilized on the surface of the spacer;
a first portion of the plurality of capture molecules that are specifically bound to target molecules;
a second portion of the plurality of capture molecules that are non-specifically bound to fluorescent detection molecules;
a first fluorophore layer comprising a plurality of fluorescent detection molecules coupled, by binding to the target molecule that are specifically bound to the first portion of the plurality of capture molecules;
a second fluorophore layer comprising a plurality of fluorescent detection molecules non-specifically bound the second portion of the plurality of capture molecules;
a light source unit configured to provide an excitation light to the substrate; and
a detection unit configured to detect a fluorescence signal emitted from a fluorophore by the excitation light;
wherein the predetermined thickness is of a thickness determined to cause constructive interference between light directly emitted from the first fluorophore layer and light emitted from the first fluorophore layer and reflected by the metal thin film, and to restrain light emitted from the second fluorophore layer.
10. The apparatus for detecting a biomaterial of claim 9 , wherein the fluorescence signal emitted from the fluorophore is excited by the excitation light and enhanced by a light reflected from the metal thin layer.
11. The apparatus for detecting a biomaterial of claim 9 , wherein the light source unit emits the excitation light in a pulse form.
12. The apparatus for detecting a biomaterial of claim 11 , wherein the fluorophore has a longer fluorescence lifetime than the pulse width of the excitation light.
13. The apparatus for detecting a biomaterial of claim 9 , wherein the detection unit detects the fluorescent signal emitted from the fluorophore through a time-resolved fluorescence measurement method.
14. The apparatus for detecting a biomaterial of claim 9 , wherein the specific binding between the capture molecule and the target molecule is selected from the group consisting of nucleic acid hybridization and enzyme binding reaction.
15. The apparatus for detecting a biomaterial of claim 9 wherein the nucleic acid comprises at least one selected from the group consisting of RNA, PNA, LNA, and a hybrid thereof.
16. The apparatus for detecting a biomaterial of claim 9 , wherein the protein comprises at least one selected from the group consisting of enzyme, matrix, antibody, ligand, aptamer, and receptor.
17. The biochip of claim 1 , wherein the substrate is formed of cyclic olefin copolymer (COC).
18. The biochip of claim 1 , wherein the metal thin film is formed of Au,
wherein the spacer is formed of an organic material,
wherein the capture molecules are immobilized by carboxyl (—COOH), which are induced on the surface of the spacer, and
wherein the fluorophore comprises a Europium (Eu 3+ ).
19. The apparatus for detecting a biomaterial of claim 9 , wherein the specific binding between the capture molecule and the target molecule comprises an antigen-antibody reaction,
wherein the capture molecule comprises a nucleic acid or a protein,
wherein the nucleic acid comprises DNA, and
wherein the protein comprises an antigen.