Compounds used as dyes comperable to Alexa Fluor 350 dyes
Compounds used as dyes comparable to Alexa Fluor 350 dyes. The inventive compounds have high fluorescence quantum yield and high photostability. The dyes facilitate analysis of biological structures with enhanced sensitivity and selectivity.
1. A compound of the formula
wherein M is selected from the group consisting of H + and a biologically compatible cation.
2. A compound of the formula
wherein M is selected from the group consisting of H + and a biologically compatible cation.
3. A compound of the formula
4. A compound of the formula
5. A compound of the formula
6. A compound of the formula
7. A method of using a compound for at least one of a bioanalytical or a diagnostic application, the method comprising contacting a biological sample with at least one of the compounds of claim 1 , 2 , 3 , 4 , 5 , or 6 , and
qualitatively detecting the compound, quantitatively detecting the compound, or both qualitatively and quantitatively detecting the compound to result in the bioanalytical application.
8. The method of claim 7 wherein the application is selected from the group consisting of fluorescence resonance energy transfer, flow cytometry, tissue detection, and combinations thereof.
9. The method of claim 7 wherein tissue detection is by hand-held ultraviolet light detection.