USE OF 2-HYDROXY-5-OXOPROLINE IN CONJUNCTION WITH ALGAE
Provided herein are exemplary methods for the use of 2-hydroxy-5-oxoproline in conjunction with algae. One exemplary method includes applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae. The effective amount of the 2-hydroxy-5-oxoproline may be approximately 0.1 grams per liter of the aqueous environment, or up to approximately 0.1 grams per liter of the aqueous environment. The effective amount of the 2-hydroxy-5-oxoproline may be applied to the aqueous environment at or near a same time, or applied to the aqueous environment over a period of time. Exemplary algae cultivation systems are also provided herein. One exemplary system includes an aqueous environment having a pale-green mutant Nannochloropsis, and an effective amount of 2-hydroxy-5-oxoproline to accelerate creation of a high-cell density of the pale-green mutant Nannochloropsis.
1 . A method for generating fuel feedstock, the method comprising:
applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae.
2 . The method of claim 1 , wherein the algae is wild-type Nannochloropsis.
3 . The method of claim 1 , wherein the algae is pale-green mutant Nannochloropsis.
4 . The method of claim 1 , wherein the algae is wild-type algae.
5 . The method of claim 1 , wherein the algae is pale-green algae established by manipulation of growth conditions of the aqueous environment.
6 . The method of claim 1 , the method further comprising treating the algae with a chemical or a genetic method to reduce an amount of chlorophyll in the algae.
7 . The method of claim 1 , wherein the algae have reduced light harvesting antennae.
8 . The method of claim 1 , wherein the algae is acclimated to high-light intensity.
9 . The method of claim 1 , wherein the effective amount of the 2-hydroxy-5-oxoproline is approximately 0.1 grams per liter of the aqueous environment.
10 . The method of claim 1 , wherein the effective amount of the 2-hydroxy-5-oxoproline is up to approximately 0.1 grams per liter of the aqueous environment.
11 . The method of claim 1 , wherein the effective amount of the 2-hydroxy-5-oxoproline is applied to the aqueous environment at or near a same time.
12 . The method of claim 1 , wherein the effective amount of the 2-hydroxy-5-oxoproline is applied to the aqueous environment over a period of time.
13 . The method of claim 1 , wherein the effective amount of the 2-hydroxy-5-oxoproline is in a range of approximately 0.1 grams per liter of the aqueous environment to approximately 0.9 grams per liter of the aqueous environment.
14 . An algae cultivation system for generating fuel feedstock, the algae cultivation system comprising:
an aqueous environment having a pale-green mutant Nannochloropsis; and
an effective amount of 2-hydroxy-5-oxoproline to accelerate creation of a high-cell density of the pale-green mutant Nannochloropsis.
15 . The algae cultivation system of claim 14 , wherein the aqueous environment includes seawater.
16 . The algae cultivation system of claim 14 , wherein the aqueous environment includes fresh water.
17 . The algae cultivation system of claim 14 , wherein the aqueous environment includes a mixture of seawater and fresh water.
18 . The algae cultivation system of claim 14 , wherein the algae cultivation system is in a photobioreactor.
19 . The algae cultivation system of claim 14 , wherein the algae cultivation system is in a pond.
20 . The algae cultivation system of claim 14 , wherein the algae cultivation system is in a vessel.