Artificial eardrum using silk protein and method of fabricating the same
Provided is an artificial eardrum using silk protein and a method of fabricating the same. The artificial eardrum is fabricated in the form of a silk membrane by desalinating and drying silk protein (or silk fibroin) or a silk protein complex solution obtained after removal of sericin from a silkworm cocoon or silk fiber. Thus, regeneration of an eardrum perforated due to disease or a sudden accident is stimulated, a boundary of the regenerated eardrum is clean and biocompatibility and transparency are increased. In addition, the artificial eardrum may be fabricated using the silk protein or silk protein complex solution obtained from a silkworm cocoon alone or mixed with collagen, alginic acid, PEG or pluronic 127.
1. An artificial eardrum comprising: a silk protein that is fabricated into a nonporous and transparent silk membrane by desalinating and drying silk protein or a silk protein complex solution obtained after removal of sericin from a silkworm cocoon or silk fiber, wherein the nonporous and transparent silk membrane is fashioned in the form of an artificial eardrum, and further includes at least one additive of gelatin, collagen, alginic acid, hyarulonic acid and pluronic 127, and has a thickness of 80-120 um, and is fabricated using the silk protein or the silk protein complex solution, which has a concentration of 0.8% to 20% at room temperature to 90° C., and the nonporous and transparent silk membrane is recrystallized using ethanol, propanol or an aqueous solution thereof.
2. The apparatus of claim 1 , wherein the silk protein or the silk protein complex solution is dissolved using a chaotropic salt composed of at least one compound of lithium bromide (LiBr), lithium chloride (LiCl 2 ), zinc chloride (ZnCl 2 ) and calcium chloride (CaCl 2 ) or an ethanol aqueous solution including the same.
3. The apparatus of claim 1 , wherein the nonporous and transparent silk membrane further includes at least one additive of a plasticizer, a softener, an antibiotic, an antibacterial agent, a cell, an enzyme, an antibody, a pigment and a combination thereof to prevent additional contamination of an applied site.
4. A method of fabricating an artificial eardrum, comprising:
(a) preparing silk protein or a silk protein complex solution after removal of sericin from a silkworm cocoon or silk fiber;
(b) desalinating and drying the prepared silk protein or silk protein complex solution to fabricate the prepared silk protein or silk protein complex solution into a nonporous and transparent silk membrane; and
(c) fashioning the nonporous and transparent silk membrane to form the artificial eardrum,
further comprising, after step (b), recrystallizing the nonporous and transparent silk membrane by solvent treatment using ethanol and propanol or an aqueous solution thereof to reduce solubility to water,
wherein the nonporous and transparent silk membrane further includes at least one additive of gelatin, collagen, alginic acid, hyarulonic acid and pluronic 127 and has a thickness of 80-120 um, and the silk protein or the silk protein complex solution has a concentration of 0.8% to 20% at room temperature to 90° C.
5. The method of claim 4 , further comprising, after step (a), dissolving the silk protein or the silk protein complex solution using a chaotropic salt composed of at least one compound of lithium bromide (LiBr), lithium chloride (LiCl 2 ), zinc chloride (ZnCl 2 ) and calcium chloride (CaCl 2 ) or an ethanol aqueous solution including the same.
6. The method of claim 4 , wherein the concentration of the silk protein or the silk protein complex solution prepared in step (a) is controlled using poly(ethylene glycol)(PEG) or by drying under reduced pressure using a drier or vacuum drier.