Process for the production of daptomycin
The present invention relates to an improved process for the production of Daptomycin by fermentation with Streptomyces roseosporus , in the presence of n-decanal or Cuphea oil as sources of the n-decanoyl side chain. These reagents allow to reduce toxicity effects on the bacteria and to avoid the use of solvents in the feeding solution.
1. Process for the production of Daptomycin, comprising fermenting Streptomyces roseosporus in the presence of n-decanal or Cuphea oil, wherein the n-decanal or Cuphea oil provide a source for a n-decanoyl side chain group.
2. The process according to claim 1 , comprising fermenting Streptomyces roseosporus in the presence of n-decanal.
3. The process according to claim 2 wherein n-decanal is provided in a solution in an organic solvent selected from the group consisting of: methyl oleate, ethanol and ethyl acetate.
4. The process according to claim 3 wherein the solvent is methyl oleate.
5. The process according to claim 2 wherein n-decanal is provided without solvents.
6. The process according to claim 1 , comprising fermenting Streptomyces roseosporus in the presence of Cuphea oil.
7. The process according to claim 6 wherein Cuphea oil is provided in a solution in an organic solvent selected from the group consisting of: methyl oleate and ethanol or mixed with soy oil, palm oil or sunflower oil.
8. The process according to claim 7 wherein the organic solvent is methyl oleate.
9. The process according to claim 6 wherein Cuphea oil is provided without solvents.
10. The process according to claim 6 which is carried out as batch fermentation with pure Cuphea oil.