Diagnosis and risk stratification by determining the marker CT-proADM
The invention relates to a novel diagnostic marker CT-proADM (C-terminal fragment of preproADM, SEQ ID No, 1) for diagnosing and/or stratifying the risk of diseases. Also disclosed is a method for diagnosing and/or stratifying the risk of diseases, particularly cardiovascular diseases, cardiac insufficiency, and infections and/or inflammations of the lungs and respiratory tract. In said method, the CT-proADM (SEQ ID No. 1) marker, or a partial peptide of fragment thereof, or said marker contained in a marker combination (panel, cluster) is determined in a patient who is to be examined. The invention further relates to a diagnostic apparatus as well as a kit for carrying out said method.
1. A method for diagnosing or stratifying risk of a disease in a patient comprising determining the level of the C-terminal fragment of proadrenomedullin (CT-proADM), as shown in SEQ ID NO: 1, or a partial peptide or fragment thereof, in a sample of body fluid or tissue from said patient, wherein the presence in said sample of an elevated level of CT-proADM in comparison with healthy controls, corresponds with the presence in said patient of said disease, wherein said disease is selected from the group consisting of interstitial pulmonary diseases, lung fibroses, chronic obstructive pulmonary diseases, and bronchial asthma.
2. The method according to claim 1 wherein the method is an in vitro method.
3. The method according to claim 1 , wherein said disease is a chronic obstructive pulmonary disease.
4. The method according to claim 1 , wherein said chronic obstructive pulmonary disease is a chronic obstructive pulmonary disease infection exacerbation or an exacerbated chronic obstructive pulmonary disease.
5. The method according to claim 1 , further comprising determining in said patient at least one additional marker selected from the group consisting of inflammatory markers, cardiovascular markers, neurohormonal markers, and ischemic markers.
6. The method according to claim 5 wherein said inflammatory markers are selected from the group consisting of C-reactive protein (CRP), cytokines, interleukins, procalcitonin (1-116, 3-116) and adhesion molecules.
7. The method according to claim 5 wherein the cardiovascular markers are selected from the group consisting of creatine kinase, kinase, myeloperoxidase, copeptin, myoglobin, a natriuretic protein, cardiac troponin, CRP, and (pro)hormones regulating circulation.
8. The method of claim 7 , wherein said natriuretic protein is selected from the group of ANP, proANP, NT-proANP, BNP, proBNP, NT-proBNP, and partial sequences thereof.
9. The method of claim 7 wherein said (pro)hormones regulating circulation are selected from the group consisting of pro-gastrin-releasing peptide (proGRP), pro-endothelin-1, pro-leptin, pro-neuropeptide-Y, pro-somatostatin, pro-neuropeptide-YY, pro-opiomelanocortin, and partial sequences thereof.
10. The method according to claim 5 wherein said ischemic markers are selected from the group consisting of troponin I and T, and CB-MB.
11. The method according to claim 5 wherein said neurohormonal markers are at least a natriuretic protein.
12. The method of claim 11 wherein said natriuretic protein is selected from the group consisting of ANP, proANP, NT-proANP, BNP, proBNP, NT-proBNP, and partial sequences thereof.