Production of Lentiviral Vectors
The present invention is a method of generating a lentivirus vector, comprising cloning each of a leotivimus transfer construct, gag, pol, an envelope protein and rev respectively into the same or different baculoviruses, and transducing a producer cell with the or each baculovirus.
1 . A method of generating a lentivirus vector, comprising cloning each of a lentivirus transfer construct, gag, pot an envelope protein and rev respectively into the same or different baculoviruses, and transducing a producer cell with the or each baculovirus.
2 . The method according to claim 1 , wherein the envelope protein is VSV-G.
3 . The method according to claim 2 , wherein the lentivirus transfer construct, gag, pol, VSV-G and rev are respectively cloned into BAC-transfer, BAC-gag-pol, BAC-VSVg and BAC-rev.
4 . The method according to claim 1 , wherein the lentivirus transfer construct, gag, pol, the envelope protein and rev are each cloned into the same baculovirus.
5 . The method, according to claim 1 , wherein the producer cell is 293T, HepG2, CHO, BHK, Sf9, Sf21, 293,BTI-Tn 5 B 1-4, COS, NIH/3T3, Vero, NSO or PerC6 cells.
6 . The method according to claim 1 , wherein the lentivirus transfer construct is a third-generation lentivirus transfer construct.