Pipette tips for extraction, sample collection and sample cleanup and methods for their use
View Patent ↗The present invention is a pipette tip device for extraction of liquid, semi-solid or solid solutions to be chemically analyzed and the methods for their use. The pipette tip extraction device contains a screen or filter at its lower narrow end to contain solid particulate matter and a barrier at its upper wide end. The optional upper frit is to be made of material that permits liquid solutions to flow through it. In addition to the barrier and screen, the pipette tip extraction device may contain solid-phase sorbent. Through the use of a removable cap, the pipette extraction tip may also serve as a sample collection container or tip in which samples can be delivered to the top of the tip (e.g., for direct collection of samples, including solid samples). A new method for DPX extraction using liquid-liquid-solid-phase extraction is also disclosed.
1. A pipette tip system, comprising:
a positive or negative pressure device;
an upper adaptor including an entry, wherein said entry is a pierceable membrane, wherein said pierceable membrane is nonporous and adapted to be pierced by said positive or negative pressure device, and wherein said upper adaptor forms an air tight seal with said positive or negative pressure device when said positive or negative pressure device pierces said pierceable membrane;
a lower liquid permeable closure;
a mixing chamber between said upper adaptor and said lower liquid permeable closure; and
sorbent materials within said mixing chamber contained loosely enough so that said sorbent materials are moveable for mixing.
2. The pipette tip as recited in claim 1 , wherein said upper adaptor includes grooves for transporting said pipette tip by an automated instrument.
3. The pipette tip in claim 1 , wherein said adaptor is removable.
4. The pipette tip in claim 1 , wherein said lower liquid permeable closure is a filtration membrane or screen.
5. The pipette tip in claim 1 , wherein said positive or negative pressure device is a syringe having a needle.
6. The pipette tip in claim 1 , further comprising an upper barrier below said adaptor.
7. A pipette tip system, comprising:
a positive or negative pressure device;
a pipette tip having an upper end and a lower end;
an adaptor at said upper end including an entry having a pierceable membrane adapted to be pierced by said positive or negative pressure device, wherein said pierceable membrane is nonporous and forms an air tight seal with said positive and negative pressure device;
a lower liquid permeable closure;
a mixing chamber between said upper adaptor and said lower liquid permeable closure; and
sorbent material contained loosely within said mixing chamber to allow for movement of said sorbent material.
8. The pipette tip in claim 7 , further comprising an upper barrier below said adaptor.
9. The pipette tip in claim 7 , wherein said positive or negative pressure device is a syringe having a needle.