METHOD FOR PREPARING NANO-EMULSIONS
The present invention relates to a method for preparing nano-emulsions comprising at least one aqueous continuous phase and at least one oily dispersed phase, comprising the steps of: (i) preparing the oily phase comprising at least one amphiphilic lipid and at least one solubilising lipid; (ii) dispersing the oily phase in an aqueous phase under the effect of sufficient shear force to form a nano-emulsion; and (iii) recovering the nano-emulsion thus formed.
1 . Method for preparing nano-emulsions comprising at least one aqueous continuous phase and at least one oily dispersed phase, comprising the steps of:
(i) preparing the oily phase comprising at least one amphiphilic lipid and at least one solubilising lipid;
(ii) dispersing the oily phase in an aqueous phase under the effect of sufficient shear force to form a nano-emulsion; and
(iii) recovering the nano-emulsion thus formed,
wherein the solubilising lipid consists of a mixture of saturated fatty acid glycerides comprising at least 10% by weight of C12 fatty acids, at least 5% by weight of C14 fatty acids, at least 5% by weight of C16 fatty acids and at least 5% by weight of C18 fatty acids.
2 . Method according to claim 1 , wherein the solubilising lipid consists of a mixture of saturated fatty acid glycerides comprising 0% to 20% by weight of C8 fatty acids, 0% to 20% by weight of C10 fatty acids, 10% to 70% by weight of C12 fatty acids, 5% to 30% by weight of C14 fatty acids, 5% to 30% by weight of C16 fatty acids and 5% to 30% by weight of C18 fatty acids.
3 . Method according to claim 1 , wherein the amphiphilic lipid is selected from phospholipids, cholesterol, lysolipids, sphingomyelins, tocopherols, glucolipids, stearylamines or cardiolipins of natural or synthetic origin.
4 . Method according to claim 1 , wherein the amphiphilic lipid is a phospholipid.
5 . (canceled)
6 . Method according to claim 1 , wherein the oily phase further comprises at least one oil.
7 . Method according to claim 6 , wherein the oil has a hydrophilic-lipophilic balance (HLB) of between 3 and 6.
8 . Method according to claim 1 , wherein the oily phase comprises at least one oil selected from soybean oil and linseed oil.
9 . Method according to claim 1 , wherein the aqueous phase further comprises a cosurfactant.
10 . Method according to claim 9 , wherein the cosurfactant comprises at least one chain composed of ethylene oxide units or ethylene oxide and propylene oxide units.
11 . Method according to claim 9 , wherein the cosurfactant is selected from the conjugated compounds polyethyleneglycol/phosphatidylethanolamine (PEG-PE), fatty acid and polyethyleneglycol ethers, fatty acid and polyethyleneglycol esters, and block copolymers of ethylene oxide and propylene oxide.
12 . Method according to claim 1 , wherein the continuous phase of the emulsion comprises a physiologically acceptable buffer.
13 . Method according to claim 1 , wherein the shear force effect is produced by sonication.
14 . Method according to claim 1 , wherein the oily phase is prepared by placing all or some of the constituents in solution in an appropriate solvent and subsequently evaporating the solvent.