Biodetection cassette with automated actuator
Provided are analysis cassettes that include removable sealing layers and deformable connector layers that place various channels, chambers, and reservoirs on the cassettes into fluid communication with one another. The cassettes are suitable for use in self-contained, immunoassay devices that may be operated in an automated fashion. Also disclosed are methods for analyzing samples by use of the disclosed cassettes.
1. An analytic device, comprising:
a cassette having a fluidic element at least partially sealed with a deformable sealing layer;
an actuator comprising an actuating plate adapted for rotational motion relative to the fluidic element, the actuating plate comprising at least one projection,
the actuator further comprising at least one moveable body contained within a housing,
the moveable body positioned between the plate and the cassette such that rotational motion of the actuating plate gives rise to the at least one projection contacting the moveable body so as to actuate the deformable sealing layer, the fluidic element, or both; and
a motivator capable of controllably moving the actuating plate relative to the deformable sealing layer.
2. The analytic device of claim 1 , wherein a fluidic element comprises a chamber, a channel, a valve, a mixer, a vent, a splitter, a switch, a reservoir, or any combination thereof.
3. The analytic device of claim 2 , wherein the reservoir contains a buffer, an acid, a base, a growth medium, a dye, a label, a reagent, a biological material, a suspension, or any combination thereof.
4. The analytic device of claim 1 , wherein the cassette comprises at least one vent to the exterior environment.
5. The analytic device of claim 1 , wherein the plate comprises a disc.
6. The analytic device of claim 5 , wherein a projection comprises a bump, a pyramid, a ramp, a wedge, or any combination thereof.
7. The analytic device of claim 1 , wherein the projection is positioned such that movement of the actuating plate effects depression of the deformable sealing layer, actuation of a fluidic element, or both, in a predetermined sequence.
8. The analytic device of claim 1 , wherein the motivator is capable of rotating the actuating plate.
9. The analytic device of claim 1 , wherein the motivator comprises a timer.
10. The analytic device of claim 1 , wherein the cassette comprises a removable film sealing a fluidic element.
11. The analytic device of claim 1 , wherein the deformable sealing layer places two or more fluidic elements in fluidic communication with one another.
12. The analytic device of claim 1 , wherein the actuator, the motivator, or both, are integrated into the cassette.
13. The analytic device of claim 2 , wherein the fluidic element is contained within a substrate.
14. The analytic device of claim 13 , wherein the substrate comprises a polymer, a glass, a metal, a ceramic, or any combination thereof.
15. The analytic device of claim 2 , wherein a channel comprises a cross-sectional dimension in the range of from about 0.01 micrometers to about 5000 micrometers.
16. The analytic device of claim 2 , wherein a fluidic element places the cassette into fluid communication with the environment exterior to the cassette.
17. The analytic device of claim 1 , wherein the cassette further comprises a lateral flow strip, a microarray, a bead array, or any combination thereof.
18. The analytic device of claim 1 , wherein the cassette defines a thickness of from about 0.5 millimeters to about 1 centimeter.
19. The analytic device of claim 1 , wherein the cassette further comprises a sealing layer and a channeled connector layer, the channeled connector layer disposed so as to place a fluidic element of the cassette in fluid communication with another fluidic element of the cassette when the sealing layer is removed and the channeled connector layer is replaced.