Methods for the detection of lung cancer
View Patent ↗Probe sets and methods of using probes and probe sets for the detection of cancer are described. Methods for detecting cancer that include hybridizing a set of chromosomal probes to a biological sample obtained from a patient, and identifying if cancer cells are present the sample. Also included are methods of selecting a combination of probes for the detection of cancer.
1. A method of screening for lung cancer in a human subject whose status with respect to lung cancer is unknown, the method comprising:
a) obtaining a biological sample, wherein the biological sample comprises a lung sample, from the subject;
b) obtaining a set of chromosomal probes consisting of two probes said two probes suitable for detecting lung cancer with greater sensitivity and specificity together than each individual probe, said set of two probes consisting of a 5p15 locus specific probe with a target location of D5S721.D5S23 and a chromosome 6 enumeration probe with a target location of D6Z1.6p11.1-q11;
c) contacting the set of probes to the biological sample under conditions sufficient to enable hybridization of probes in the set to chromosomes in the sample, if any; and
d) detecting a hybridization pattern consisting of gains or losses of copy number of chromosomal target regions as determined by the set of chromosomal probes hybridized to the chromosomes in the biological sample to determine whether the subject has lung cancer.
2. The method of claim 1 , wherein the biological sample consists of one or more of a bronchial specimen, a lung biopsy, or a sputum sample.
3. The method of claim 1 , wherein the chromosomal probes are fluorescently labeled.
4. The method of claim 1 , further comprising performing cytological analysis on the sample.