Ubiquitin regulatory elements
View Patent ↗The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a constitutive regulatory element isolated from sorghum. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
1. An isolated regulatory element that drives transcription in a constitutive manner, wherein the regulatory element comprises
the nucleotide sequence of SEQ ID NO: 1.
2. An expression cassette comprising a regulatory element operably linked to a nucleotide sequence wherein the regulatory element comprises the nucleotide sequence of claim 1 .
3. A plant stably transformed with an expression cassette of claim 2 .
4. The plant of claim 3 , wherein said plant is a monocot.
5. The plant of claim 3 , wherein said monocot is maize, wheat, rice, barley, sorghum or rye.
6. Seed of the plant of claim 3 wherein the seed comprises the expression cassette.
7. A method for selectively expressing a nucleotide sequence in a plant cell, the method comprising:
a) transforming a plant cell with an expression cassette, the expression cassette comprising a regulatory element operably linked to a nucleotide sequence wherein the regulatory element comprises
the nucleotide sequence set forth in SEQ ID NO: 1;
b) growing the plant cell to selectively express the nucleotide sequence.
8. The method of claim 7 wherein the regulatory element initiates expression of the nucleotide sequence in plant tissue.
9. The method of claim 7 further comprising regenerating a stably transformed plant from the plant cell; wherein expression of the nucleotide sequence alters the phenotype of a plant.
10. The plant of claim 9 , wherein said plant is a monocot.
11. The plant of claim 9 , wherein said monocot is maize, wheat, rice, barley, sorghum or rye.
12. Seed of the plant of claim 9 wherein the seed comprises the expression cassette.