MAMMALIAN ZCYTOR 11
Novel receptor polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. The polypeptides comprise an extracellular domain of a cell-surface receptor that is expressed in pancreas, small intestine, colon and thymus. The polypeptides may be used within methods for detecting ligands that promote the proliferation and/or differentiation of these organs.
1 . An isolated polynucleotide encoding a polypeptide, wherein the encoded polypeptide comprises amino acid residues 18 to 228 of SEQ ID NO:2.
2 . The isolated polynucleotide of claim 1 , wherein the polynucleotide comprises nucleotides 85 to 717 of SEQ ID NO:1.
3 . The isolated polynucleotide of claim 1 , wherein the encoded polypeptide comprises the amino acid sequence of SEQ ID NO:2.
4 . The isolated polynucleotide of claim 1 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:1.
5 . The isolated polynucleotide of claim 1 , wherein the encoded polypeptide further comprises a transmembrane domain.
6 . The isolated polynucleotide of claim 5 , wherein the transmembrane domain comprises amino acid residues 229 to 251 of SEQ ID NO:2.
7 . The isolated polynucleotide of claim 1 , wherein the encoded polypeptide further comprises an intracellular domain.
8 . The isolated polynucleotide of claim 7 , wherein the intracellular domain comprises amino acid residues 252 to 574 of SEQ ID NO:2.
9 . The isolated polynucleotide of claim 1 , wherein the encoded polypeptide further comprises an affinity tag.
10 . The isolated polynucleotide of claim 9 , wherein the affinity tag is selected from the group consisting of polyhistidine, protein A, glutathione S transferase, substance P, and an immunoglobulin heavy chain constant region.
11 . An isolated polynucleotide encoding a polypeptide selected from the group consisting of:
amino acid residues 1 to 228 of SEQ ID NO:2;
amino acid residues 1 to 251 of SEQ ID NO:2;
amino acid residues 1 to 574 of SEQ ID NO:2;
amino acid residues 18 to 228 of SEQ ID NO:2;
amino acid residues 18 to 251 of SEQ ID NO:2;
amino acid residues 18 to 574 of SEQ ID NO:2;
amino acid residues 229 to 251 of SEQ ID NO:2;
amino acid residues 229 to 574 of SEQ ID NO:2; and
amino acid residues 252 to 574 of SEQ ID NO:2.
12 . An expression vector comprising the following operably linked elements:
a transcription promoter;
a DNA segment encoding a polypeptide, wherein the encoded polypeptide comprises amino acid residues 18 to 228 of SEQ ID NO:2; and
a transcription terminator.
13 . The expression vector of claim 12 , wherein the encoded polypeptide further comprises a signal sequence.
14 . The expression vector of claim 12 , wherein the encoded polypeptide further comprises a transmembrane domain.
15 . The expression vector of claim 14 , wherein the transmembrane domain comprises amino acid residues 229 to 251 of SEQ ID NO:2.
16 . The expression vector of claim 12 , wherein the encoded polypeptide further comprises an intracellular domain.
17 . The expression vector of claim 16 , wherein the intracellular domain comprises amino acid residues 252 to 574 of SEQ ID NO:2.
18 . A transformed or transfected cell into which has been introduced an expression vector according to claim 12 , wherein the cell expresses the encoded polypeptide.