Amphiphilic polymer capsules and related methods of interfacial assembly
Polymer capsules from amphiphilic graft copolymers comprising reactive, hydrophobic polyolefin backbones, and hydrophilic poly(ethylene glycol) (PEG) grafts are produced by self-assembly of the polymers at the oil-water interface, and crosslinking the assembly with bis-cyclooctene PEG derivatives in conjunction with ring-open metathesis polymerization catalysts. The use of the graft copolymer architecture in capsule synthesis provides significant opportunities to tune both the surface properties, in terms of recognition, and the membrane properties, in terms of mechanical strength, encapsulation, and release.
1. A method of using a graft co-polymer for interfacial assembly, said method comprising:
providing first and second fluid phase components, said fluid phase components at least partially immiscible one with the other, and providing a fluid interface;
providing a first cycloalkene monomeric component, said component comprising a pendent poly(alkylene oxide) substituent, said substituent interactive with one of said fluid phase components; and
polymerizing said monomeric component, said polymer assembled at said fluid interface.
2. The method of claim 1 comprising a second cycloalkene monomeric component.
3. The method of claim 2 wherein said second cycloalkene monomeric component comprises a pendent functionality for cross-linking with another monomeric component.
4. The method of claim 2 wherein said second cycloalkene monomeric component comprises a bis-cycloalkene component, said cycloalkene moieties linked with a poly(alkylene oxide) moiety.
5. The method of claim 2 comprising a third cycloalkene monomeric component comprising a pendent functionality for cross-linking with another said third monomeric component.
6. The method of claim 1 wherein at least one of said fluid phase components is removed after polymerization.
7. The method of claim 1 wherein an agent is partitioned in one of said fluid phase components.
8. The method of claim 7 wherein at least one of said phases is removed after polymerization.
9. The method of claim 1 wherein said first monomeric component comprises cyclooctene, and said pendent substituent comprises poly(ethylene oxide).
10. The method of claim 9 comprising at least one of a cyclooctene monomeric component, a bis-cyclooctene monomeric component comprising a poly(alkylene oxide) cross-linking moiety, and a cyclooctene monomeric component comprising a pendent functionality for cross-linking.
11. The method of claim 10 wherein said pendent functionality is selected from methacrylate, methacrylamide, acetylene and azide.
12. The method of claim 9 wherein said first fluid phase component is selected from aqueous, protic and polar components, and said second fluid phase component is at least partially immiscible therewith.
13. The method of claim 12 wherein an agent is partitioned in said second fluid phase component, said agent selected from therapeutic, analytic and diagnostic agents.
14. The method of claim 13 comprising removing said first fluid phase component, said agent within said polymer assembly.