Product and method for producing an immune system supplement and performance enhancer

1. A method for producing a balanced foundational redox-signaling oral (RSO) compound to enhance the efficiency and production of the body's native antioxidants and the performance of intercellular communications involved in the maintenance of healthy tissues and athletic performance, comprising:

a. determining a balanced target mixture of chemical redox-signaling molecules for healthy cells and measuring concentrations of reactive molecules present using a combination of R-Phycoerythrin (R-PE), Aminophenyl Fluorescein (APF) and Hydroxyphenyl Fluorescein (HPF),

b. electrochemically replicating this target mixture by:

i. mixing an initial circulating saline solution,

ii. adjusting the temperature, flow and sodium chloride concentration of the circulating saline solution at a level to prevent production of chlorates and regulate relative concentrations of resulting reactive molecules,

iii. activating an electrochemical device to oxidize and reduce the circulating saline solution to produce a concentration and mixture of reactive molecules similar to those present in the target mixture by varying temperature, flow, pH, power-source modulation, salt homogeneity, and concentration parameters of the circulating saline solution to form an RSO compound, and

c. verifying the similarity of the concentration of the redox-signaling molecules in the target mixture with those in the electrochemically replicated RSO compound to produce a verified RSO compound by using the combination of R-Phycoerythrin (R-PE), Aminophenyl Fluorescein (APF) and Hydroxyphenyl Fluorescein (HPF) used to measure the concentrations of reactive molecules present in the target mixture.

2. A method for producing a balanced foundational redox-signaling oral compound according to claim 1 , wherein the combination of R-Phycoerythrin (R-PE), Aminophenyl Fluorescein (APF) and Hydroxyphenyl Fluorescein (HPF) is used for verification such that:

i. a 2 micromolar concentration of R-PE loses 5%-50% of its fluorescence 6 hours after a 1:1000 solution of the RSO compound is added,

ii. R-PE measurements indicate the same fluoroescence levels as a standard ROS generating solution of 0.2 or 1.0 mM AAPH,

iii. APF measurements indicate the same relative amount as the target solution,

iv. HPF measurements indicate a negligibly small reading,

v. a pH of between 7.2 and 7.5, and

vi. total chlorine concentration of less than 35 ppm by weight.

3. A method for producing a balanced foundational redox-signaling oral compound according to claim 1 , including activating a fluid circulation device to maintain flow aging stratification and homogeneity of the circulating saline solution.

4. A method for producing a balanced foundational redox signaling oral compound according to claim 1 , wherein the circulating saline solution has approximately 0.05% to 10% sodium chloride concentration by weight.

5. A method for producing a balanced foundational redox-signaling oral compound according to claim 1 , wherein the combination of Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) is used for the detection of highly reactive oxygen species (hROS).

6. A method for enhancing an athletic performance characteristic, comprising:

administering an RSO compound formed by activating an electrochemical device to oxidize and reduce a circulating saline solution to produce a concentration and mixture of reactive molecules similar to those present in a target mixture by varying temperature, flow, pH, power-source modulation, salt homogeneity, and concentration parameters of the circulating saline solution to form the RSO compound; and

enhancing an athletic performance characteristic, wherein the method further comprises verifying the similarity of the concentration of the redox-signaling molecules in the target mixture with those in the RSO compound by analyzing measurements from a combination of R-Phycoerythrin (R-PE), Aminophenyl Fluorescein (APF) and Hydroxyphenyl Fluorescein (HPF) assays.

7. The method according to claim 6 , wherein the measurements from the combination of R-Phycoerythrin (R-PE), Aminophenyl Fluorescein (APF) and Hydroxyphenyl Fluorescein (HPF) assays are used for verification such that:

i. a 2 micromolar concentration of R-PE loses 5%-50% of its fluorescence 6 hours after a 1:1000 solution of the RSO compound is added,

ii. R-PE measurements indicate the same fluoroescence levels as a standard ROS generating solution of 0.2 or 1.0 mM AAPH,

iii. APF measurements indicate the same relative amount as the target solution,

iv. HPF measurements indicate a negligibly small reading,

v. a pH of between 7.2 and 7.5, and

vi. total chlorine concentration of less than 35 ppm by weight.

8. The method according to claim 6 , including activating a fluid circulation device to maintain flow aging stratification and homogeneity of the circulating saline solution.

9. The method according to claim 6 , wherein the circulating saline solution has approximately 0.05% to 10% sodium chloride concentration by weight.

10. The method according to claim 6 , wherein the combination of Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) is used for detection of highly reactive oxygen species (hROS).

11. The method according to claim 6 , wherein the athletic performance characteristic is higher power output, longer time to ventilatory threshold, longer time to VO 2max , or increased efficiency of cells during athletic performance.