Method and device for continuous membrane adsorption
A method and system is provided for yielding biopharmaceutical products involving a chromatographic separation process. The method comprises: providing a plurality of membrane adsorber cartridges; providing a plurality of valves, communicatively coupled to said plurality of membrane adsorber cartridges; and switching the valves, so as to interconnect said membrane adsorber cartridges to operate in a countercurrent flow mode. The system comprises multiple membrane adsorber cartridges that are interconnected and configured to operate in a countercurrent flow mode. Furthermore, the configuration comprises a valve assembly that allows the cartridges to be subjected to different steps in the process by automatic switching of the valves. In this way, cartridges are recycled many times during the purification of a batch.
1. A method for providing a biopharmaceutical product involving a chromatographic separation process, the method comprising:
providing a plurality of membrane adsorber cartridges;
providing a plurality of valves, communicatively coupled to said plurality of membrane adsorber cartridges, at least some of the cartridges being connected in series; and
switching the valves, so as to subsequently subject said membrane adsorber cartridges to a number of process steps of a process cycle; to thereby operate in a countercurrent flow mode;
performing a binding action including providing a process fluid in at least one of said plurality of membrane adsorber cartridges for binding a component of interest to a membrane adsorber provided in said at least one membrane adsorber cartridge; and
performing an eluting action including providing an elution fluid flow in said at least one adsorber cartridge to elute a product of interest from the at least one membrane adsorber; and
providing the product of interest as the biopharmaceutical product; wherein
a first switching action including switching at least one first valve of the valve assembly initiates the elution action; and wherein
a second switching action including switching at least one second valve of the valve assembly initiates said binding action; and wherein
the first valve and the second valve are switched asynchronously, thereby controlling the residence time of the membrane adsorber cartridge in each zone individually, and
wherein said eluting action further includes:
a washing action including providing a buffer in said membrane adsorber cartridge to displace said process fluid from a dead volume in said at least one membrane adsorber cartridge by said buffer; followed by
a fourth switching action including switching at least one valve of the valve assembly to initiate providing said elution fluid flow to elute the product of interest.
2. A method according to claim 1 , wherein said binding action further includes;
a regenerating action including providing at least one regenerating fluid in said at least one adsorber cartridge to the membrane adsorbers; followed by
a third switching action including switching at least one valve of the valve assembly to initiate said providing of said process fluid for binding a component of interest.
3. A method according to claim 2 wherein said at least one regenerating fluid has functionality to clean, sanitize and/or strip and/or equilibrate the membrane adsorbers prior to binding the component of interest.
4. A method according to claim 1 , wherein said plurality of valves is combined in at least one modular disposable format valve cassette.
5. A method according to claim 1 , wherein said membrane adsorber cartridge has adsorber functionality using affinity chromatography, ion exchange chromatography and/or mixed mode chromatography to bind and elute a product of interest.
6. The method according to claim 5 wherein the steps are performed to purify recombinant protein products.
7. A method according to claim 1 , wherein said membrane adsorber cartridge has adsorber functionality using protein A chromatography, cation exchange and/or mixed mode ligands.
8. The method according to claim 7 wherein the steps are performed to purify monoclonal antibodies.
9. A method according to claim 1 , wherein said membrane adsorber cartridge has adsorber functionality using affinity chromatography, ion exchange or mixed mode ligands.
10. The method according to claim 9 wherein the steps are performed to purify DNA or viral vectors.
11. A method according to claim 1 , wherein said process steps include, in an iterative way:
performing a binding action including providing a process fluid in at least one of said plurality of membrane adsorber cartridges for binding a group of contaminants to a membrane adsorber provided in said at least one membrane adsorber cartridge; and
performing a regenerating action including providing at least one regenerating fluid in said at least one adsorber cartridge to the membrane adsorbers prior to the binding action, wherein a first switching action including switching at least one valve of the valve assembly initiates said regenerating action; and wherein a second switching action including switching at least one valve of the valve assembly initiates said binding action.
12. A method according to claim 11 , wherein at least one regenerating fluid has desorbing functionality to desorb a contaminant from the at least one membrane adsorber.
13. A method according to claim 11 wherein said at least one regenerating fluid has functionality to clean, sanitize and/or strip and/or equilibrate the membrane adsorbers prior to binding the component of interest.
14. The method according to claim 11 wherein the steps are performed to polish monoclonal antibodies, recombinant proteins, viral vectors or DNA products.
15. The method of claim 1 wherein, after initiating the elution action by performing the first action, the second switching action is performed to repeat the binding action until a stopping criterion is reached, and wherein the stopping criterion is taken from the group consisting of: a time period elapses, and a concentration of a specific product is reached.