Detoxifying pre-treated lignocellulose-containing materials
The invention relates to a process of detoxifying pre-treated lignocellulose-containing material comprising subjecting the pre-treated lignocellulose-containing material to one or more phenolic compound oxidizing enzymes.
1. A process for producing a fermentation product, comprising:
(a) pre-treating a lignocellulose-containing corn stover or corn fiber material that contains between 30-60 wt. % lignocellulose, with dilute-acid catalyzed steam explosion, to produce a pre-treated lignocellulose-containing material;
(b) separating said pre-treated material of step (a), into a liquor fraction and solids fraction after pre-treating and then detoxifying the separated liquor fraction, unwashed, with a phenolic compound oxidizing enzyme a laccase (EC 1.10.3.2), to produce a detoxified material;
(c) after step (b), hydrolyzing the detoxified material with a cellulolytic enzyme preparation comprising a cellulase and a polypeptide having cellulolytic enhancing activity that is a family GH61A polypeptide, to produce a sugar;
(d) fermenting the sugar in a fermentation medium/broth using a fermenting yeast, to produce a fermentation product comprising an alcohol; and
(e) separating the alcohol of step (d) from the fermentation medium/broth.
2. The process of claim 1 , wherein the hydrolysis step (c) and the fermentation step (d), are carried out as a simultaneous saccharification and fermentation.
3. The process of claim 1 , wherein the fermentation product is ethanol.
4. The process of claim 1 , wherein the cellulolytic enzyme preparation is derived from Trichoderma reesei.
5. The process of claim 1 , wherein the hydrolysis step (c) and the fermentation step (d), are carried out as a hybrid hydrolysis and fermentation.