Simian T-cell lymphotropic virus
Disclosed are the simian T-cell lymphotropic virus type 3 subtype D (STLV-3 subtype D), isolated nucleic acid molecules encoding STLV-3 subtype D polypeptides, such as STLV-3 subtype D envelope, protease, polymerase, tax, rex, and capsid polypeptides, isolated polypeptides encoded by such nucleic acids. Methods are also disclosed for detecting STLV-3 subtype D, for example by detecting a STLV-3 subtype D nucleic acid or polypeptide in the sample. Accordingly, probes, primers, and antibodies for use in detecting STLV-3 subtype D nucleic acids or polypeptides are disclosed. Therapeutic compositions which included isolated nucleic acid molecules encoding a STLV-3 subtype D polypeptides or isolated polypeptides encoded by such nucleic acid molecules are also disclosed.
1. An isolated nucleic acid molecule encoding a STLV-3 subtype D (simian T-cell lymphotropic virus type 3 West African subtype D) polypeptide comprising a nucleic acid sequence at least 95% identical to one of: nucleotides 747-2009 of SEQ ID NO: 1; nucleotides 1961-2494 of SEQ ID NO: 1; nucleotides 2416-5061 of SEQ ID NO: 1; nucleotides 5054-6535 of SEQ ID NO: 1; SEQ ID NO: 25; or SEQ ID NO: 26; and a heterologous promoter; wherein the isolated nucleic acid molecule is operably linked to the heterologous promoter.
2. A vector comprising the nucleic acid molecule of claim 1 .
3. An isolated host cell transformed with the vector of claim 2 .
4. The vector of claim 2 , wherein the vector is a viral vector.
5. An isolated viral particle comprising the viral vector of claim 4 .
6. The isolated nucleic acid molecule of claim 1 , wherein the isolated nucleic acid molecule encoding a STLV-3 subtype D (simian T-cell lymphotropic virus type 3 West African subtype D) polypeptide comprises a nucleic acid sequence 100% identical to one of: nucleotides 747-2009 of SEQ ID NO: 1; nucleotides 1961-2494 of SEQ ID NO: 1; nucleotides 2416-5061 of SEQ ID NO: 1; nucleotides 5054-6535 of SEQ ID NO: 1; SEQ ID NO: 25; or SEQ ID NO: 26.
7. The isolated nucleic acid molecule of claim 1 , wherein the isolated nucleic acid molecule encoding a STLV-3 subtype D (simian T-cell lymphotropic virus type 3 West African subtype D) polypeptide consists of a nucleic acid sequence according to one of: nucleotides 747-2009 of SEQ ID NO: 1; nucleotides 1961-2494 of SEQ ID NO: 1; nucleotides 2416-5061 of SEQ ID NO: 1; nucleotides 5054-6535 of SEQ ID NO: 1; SEQ ID NO: 25; or SEQ ID NO: 26.
8. An isolated nucleic acid molecule comprising an isolated STLV-3 subtype D virus comprising a nucleic acid sequence at least 95% identical to SEQ ID NO: 1 and a heterologous promoter, wherein the isolated nucleic acid molecule is operably linked to the heterologous promoter.