System for delivering therapeutic agents into living cells and cells nuclei
The present invention relates to a novel delivery system for delivering therapeutic agents into living cells, and more particularly, to novel chemical moieties that are designed capable of targeting and/or penetrating cells or other targets of interest and further capable of binding therapeutic agents to be delivered to these cells, and to delivery systems containing same.
1. An oligomer represented by the structure of Formula I:
wherein:
n is an integer between 2 to 10;
each of L 1 -L n is independently a first linking group;
each Y is independently a delivering group or absent, provided that at least one of Y is a delivering group, wherein said delivering group is selected from the group consisting of an amine, histidine, guanidine, polyguanidine, imidazole and polyimidazole;
each of F is independently, nothing, or phosphate and
each of Z 1 and Z 2 is independently a reactive group capable of binding a biologically active moiety or absent, provided that at least one of Z 1 and Z 2 is a reactive group wherein said reactive group is hydroxy, amine, halide, a phosphorous-containing group, phosphoramidite, C-amide, N-amide, thiol, COOH or optionally a protected reactive group;
wherein said L 1 -L n are independently a substituted or unsubstituted hydrocarbon alkyl chain or a substituted or unsubstituted hydrocarbon alkyl chain optionally interrupted by at least one heteroatom, said heteroatom is an oxygen, nitrogen, sulfur or any combination thereof;
wherein w, w′ and w″ are each independently between 1 to 6.
2. The oligomer of claim 1 , wherein at least one of said reactive groups (Z1-Zn) is a protected reactive group.
3. The oligomer of claim 1 , wherein said biologically active moiety is a therapeutically active agent, a labeling moiety, or any combination thereof;
wherein said therapeutically active agent is an oligonucleotide, a nucleic acid construct, an antisense, a plasmid, a polynucleotide, an amino acid, a peptide, a polypeptide, a hormone, a steroid, an antigen, a radioisotope, a chemotherapeutic agent, a toxin, an anti-inflammatory agent, a growth factor and any combination thereof; and
wherein said labeling moiety is a chromophore, a fluorescent moiety, a radiolabeled moiety, a phosphorescent moiety, a heavy metal cluster moiety or any combination thereof.
4. The oligomer of claim 1 , represented by the following structure:
wherein w, w′ and w″ are each independently between 1 to 6;
t is between 1 to 8;
Y, L 1 -L n , F and Z 1 and Z 2 are as defined in claim 1 .
5. The oligomer of claim 4 , wherein Y is guanidine.
6. The oligomer of claim 4 , represented by the following structure:
7. A conjugate comprising the oligomer of claim 1 and at least one biologically active moiety, wherein said biologically active moiety is attached to the oligomer through Z 1 or Z 2 , said biologically active moiety is a therapeutically active agent, a labeling moiety, or any combination thereof;
wherein said therapeutically active agent is an oligonucleotide, a nucleic acid construct, an antisense, a plasmid, a polynucleotide, an amino acid, a peptide, a polypeptide, a hormone, a steroid, an antigen, a radioisotope, a chemotherapeutic agent, a toxin, an anti-inflammatory agent, a growth factor and any combination thereof; and
wherein said labeling moiety is a fluorescent moiety, a radiolabeled moiety, a phosphorescent moiety, a heavy metal cluster moiety or any combination thereof.
8. The conjugate of claim 7 , wherein said biologically active moiety is an oligonucleotide, RNA or DNA.
9. A method of delivering a biologically active moiety to a cell, said method comprises: contacting said cell with the conjugate of claim 7 , thereby delivering the biologically active moiety to the cell.
10. A pharmaceutical composition comprising the conjugate of claim 7 and a pharmaceutically acceptable carrier.
11. The oligomer of claim 1 , wherein Y is guanidinde.