Systems and methods for extracting lipids from and dehydrating wet algal biomass
View Patent ↗Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.
1. A method comprising:
centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass;
mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture;
heating the mixture to result in a dehydrated, defatted algal biomass;
separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids;
evaporating the amphiphilic solvent from the water and the lipids; and
separating the water from the lipids.
2. The method of claim 1 , wherein the amphiphilic solvent is selected from the group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde.
3. The method of claim 1 , wherein the mixture is heated in a pressurized reactor.
4. The method of claim 3 , wherein the pressurized reactor is a batch or a continuous pressurized reactor.
5. The method of claim 1 , wherein the mixture is heated with microwaves, ultrasound, steam, or hot oil.
6. The method of claim 1 , wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via membrane filtration to result in amphiphilic solvent, water and lipids.
7. The method of claim 1 , wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via centrifugation to result in amphiphilic solvent, water and lipids.
8. The method of claim 1 , wherein the separating includes decanting the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids.
9. The method of claim 1 , wherein the separating of the water from the lipids includes adding a nonpolar solvent.
10. The method of claim 9 , wherein the nonpolar solvent is propane, butane, pentane, hexane, butene, propene, naphtha or gasoline.
11. A method comprising:
filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass;
mixing the filtered algal biomass with an amphiphilic solvent to result in a mixture;
heating the mixture to result in a dehydrated, defatted algal biomass;
separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids;
evaporating the amphiphilic solvent from the water and the lipids; and
separating the water from the lipids.
12. The method of claim 11 , wherein the wet algal biomass is filtered to increase the solid content to approximately 30%.
13. The method of claim 11 , wherein the amphiphilic solvent is selected from the group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde.
14. The method of claim 11 , wherein the mixture is heated in a pressurized reactor.
15. The method of claim 14 , wherein the pressurized reactor is a batch or a continuous pressurized reactor.
16. The method of claim 11 , wherein the mixture is heated with microwaves, ultrasound, steam, or hot oil.
17. The method of claim 11 , wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via membrane filtration to result in amphiphilic solvent, water and lipids.
18. The method of claim 11 , wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via centrifugation to result in amphiphilic solvent, water and lipids.
19. The method of claim 11 , wherein the separating includes decanting the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids.
20. The method of claim 11 , wherein the separating of the water from the lipids includes adding a nonpolar solvent.