Method and device for measuring multiple physiological properties of cells
A method of analyzing cells disposed in media within a vessel includes the steps of providing a vessel having an original volume of media about the cells, reducing the original volume of media about at least a portion of the cells to define a reduced volume of media, and analyzing a constituent related to the cells within the reduced volume of media. An apparatus for analyzing cells includes a stage adapted to receive a vessel holding cells and a volume of media, a plunger adapted to receive a barrier to create a reduced volume of media within the vessel including at least a portion of the cells, the barrier adapted for insertion into the vessel by relative movement of the stage and the plunger, and a sensor in sensing communication with the reduced volume of media, wherein the sensor is configured to analyze a constituent disposed within the reduced volume.
1. An apparatus for analysis of cells disposed in media in multiple wells of a multi-well plate, the apparatus comprising:
a plurality of barriers for insertion into respective wells of the multi-well plate, each barrier comprising a barrier surface that creates, when inserted into the respective well, a sample chamber having a reduced volume of medium less than 50% of the original volume of medium in the wells by relative movement of the barriers and the multi-well plate, and
disposed on barrier surfaces, fluorescent sensors for analyzing a constituent of the medium disposed about the cells in the respective sample chamber.
2. The apparatus of claim 1 wherein the plurality of barriers comprises 24 barriers defining an array.
3. The apparatus of claim 1 wherein the plurality of barriers comprises 96 barriers defining an array.
4. The apparatus of claim 1 wherein the fluorescent sensors sense an oxygen concentration in the medium.
5. The apparatus of claim 1 wherein the fluorescent sensors sense a hydrogen ion concentration in the medium.
6. The apparatus of claim 1 wherein the plurality of barriers defines a removable cover.
7. The apparatus of claim 6 wherein the removable cover is disposable.
8. The apparatus of claim 1 wherein each barrier has a distal end, and the sensors are disposed on barrier surfaces of the respective distal ends.