Methods and products related to the improved analysis of carbohydrates
View Patent ↗The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins and saccharides attached to lipids. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information from the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation. Such methods are also provided. Methods are further provided to assess production processes, to assess the purity of samples containing glycoconjugates, and to select glycoconjugates with the desired glycosylation.
1. A system for identifying or comparing a sample comprising one or more glycans, the system comprising:
(i) a database, tangibly embodied in a non-transitory computer-readable medium, containing information descriptive of a population of glycoconjugates that each comprise a glycan covalently linked to a non-saccharide moiety, the database comprising:
one or more data units corresponding to the population of glycoconjugates, each of the data units including an identifier that includes three or more fields, each field for storing a value corresponding to one or more properties of the population of glycoconjugates, wherein the properties comprise:
a. the total mass of glycans in the population of glycoconjugates; and
b. the mass of a glycoconjugate in the population of glycoconjugates;
and wherein the properties further comprise one or more properties selected from the group consisting of:
c. the occupancy of one or more glycosylation sites in the population of glycoconjugates;
d. the linkage of one or more glycans in the population of glycoconjugates; and
e. the linkage abundance of one or more glycans in the population of glycoconjugates; and
(ii) a feature extractor adapted to access the database and compare values therein with those from the sample, so that datasets are generated and pattern analysis or data mining is performed to generate or analyze patterns, relationships or rules between or among values within the database and those from the sample.
2. The system of claim 1 wherein the population of glycoconjugates is produced in a mammalian cell.
3. The system of claim 2 , wherein the mammalian cell is a CHO cell.
4. The system of claim 1 , wherein a glycome pattern of the glycoconjugates is determined.
5. The system of claim 4 , wherein the glycome pattern is used to determine sample purity.
6. The system of claim 1 , wherein the properties comprise:
a. the total mass of glycans in the population of glycoconjugates;
b. the mass of a glycoconjugate in the population of glycoconjugates;
c. the occupancy of one or more glycosylation sites in the population of glycoconjugates; and
d. the linkage of one or more glycans in the population of glycoconjugates.
7. The system of claim 1 , wherein the properties comprise:
a. the total mass of glycans in the population of glycoconjugates;
b. the mass of a glycoconjugate in the population of glycoconjugates;
c. the occupancy of one or more glycosylation sites in the population of glycoconjugates; and
e. the linkage abundance of one or more glycans in the population of glycoconjugates.
8. The system of claim 1 , wherein the properties comprise:
a. the total mass of glycans in the population of glycoconjugates;
b. the mass of a glycoconjugate in the population of glycoconjugates;
c. the occupancy of one or more glycosylation sites in the population of glycoconjugates;
d. the linkage of one or more glycans in the population of glycoconjugates; and
e. the linkage abundance of one or more glycans in the population of glycoconjugates.
9. The system of claim 1 , wherein the patterns, relationships or rules are associated with one or more samples of known origin.
10. The system of claim 1 , wherein the sample is from a subject and the pattern provides information about the subject's state.
11. The system of claim 1 , wherein the pattern is compared to another pattern.
12. The system of claim 1 , wherein the pattern is associated with the prognosis of a disease.
13. The system of claim 1 , wherein the pattern is associated with a drug treatment.
14. The system of claim 4 , wherein one or more features of the glycoprofile is extracted and analyzed such that the glycome pattern of the sample is validated.