Materials and methods for efficient lactic acid production
The present invention provides derivatives of Escherichia coli constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.
1. A genetically modified E. coli strain comprising the following modifications:
i) inactivation or deletion of an acetate kinase gene;
ii) inactivation or deletion of a fumarate reductase gene;
iii) inactivation or deletion of a pyruvate formatelyase gene;
iv) inactivation or deletion of an alcohol dehydrogenase gene;
v) inactivation or deletion of a methylglyoxal synthase gene; and
vi) insertion of a heterologous L-(+) lactate dehydrogenase (ldhL) gene.
2. The genetically modified E. coli stain according to claim 1 , wherein said heterologous L-(+) lactate dehydrogenase gene replaces the native ldhA gene encoding a D-(−) lactate dehydrogenase.
3. The genetically modified E. coli strain according to claim 1 , wherein said heterologous L-(+) lactate dehydrogenase gene is derived from Pediococcus Acidilactici.
4. The genetically modified E. coli strain according to claim 1 , wherein said E. coli strain is selected from a group consisting of TG105, TG106, TG107 and TG108.
5. A method of producing lactate or lactic acid comprising culturing the genetically modified E. coli strain according to claim 1 under conditions that allow for the production of lactic acid and optionally neutralizing the lactic acid to form lactate.
6. The method according to claim 5 , wherein said method produces L-(+)-lactate or L-(+)-lactic acid with a chiral purity of at least 99.9%.
7. The method according to claim 5 , wherein lactate or lactic acid is produced at concentrations of at least 0.5M.
8. A composition comprising culture medium and a genetically modified E. coli strain according to claim 1 .