Patent
Streptococcus proteins and polynucleotides encoding them are disclosed. Said proteins are antigenic and therefore useful vaccine components for the prophylaxis or therapy of streptococcus infection in animals. Also disclosed are recombinant methods of producing the protein antigens as well as diagnostic assays for detecting streptococcus bacterial infection.
1. An isolated polynucleotide encoding a polypeptide consisting of an amino acid sequence at least 95% identical to the full-length amino acid sequence of selected from the group consisting of SEQ ID NO: 10, 64, 65, and 66.
2. The isolated polynucleotide of claim 1 , wherein the encoded polypeptide consists of the amino acid sequence selected from the group consisting of SEQ ID NO: 10, 64, 65, and 66.
3. An isolated polynucleotide that is the complement of the polynucleotide of claim 1 .
4. The polynucleotide of claim 1 , wherein said polynucleotide is DNA.
5. The polynucleotide of claim 1 , wherein said polynucleotide is RNA.
6. A vector comprising the polynucleotide of claim 1 , wherein the polynucleotide is operably linked to an expression control region.
7. An isolated host cell transfected with the vector of claim 6 .
8. A process for producing a polypeptide, said process comprising culturing the host cell according to claim 7 under conditions suitable for expression of the polypeptide.
9. The isolated polynucleotide according to claim 1 , wherein the encoded polypeptide elicits antibodies that specifically bind to a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:2, and wherein the encoded polypeptide is capable of inducing an immune response to Streptococcus.
10. The isolated polynucleotide according to claim 9 , wherein the Streptococcus is S. pneumoniae , group A streptococcus ( S. pyogenes ), group B streptococcus ( S. agalactiae ), S. dysgalactiae , or S. uberis.
11. The polynucleotide according to claim 10 , wherein the Streptococcus is S. pneumoniae.
12. An isolated polynucleotide encoding a chimeric polypeptide that comprises two or more polypeptides, wherein the amino acid sequence of each of the two or more polypeptides is at least 95% identical to the full-length amino acid sequence selected from the group consisting of SEQ ID NO:2, 8, 10, 16, 55, 64, 65, 66, and 78, provided that the polypeptides are linked to form the chimeric polypeptide.
13. The isolated polynucleotide according to claim 12 , wherein the amino acid sequence of each of the two or more polypeptides is selected from the group consisting of SEQ ID NO:2, 8, 10, 16, 55, 64, 65, 66, and 78.
14. A vector comprising the polynucleotide of claim 12 , wherein the polynucleotide is operably linked to an expression control region.
15. An isolated host cell transfected with the vector of claim 14 .
16. A process for producing a chimeric polypeptide, said process comprising culturing the host cell according to claim 15 under conditions suitable for expression of the chimeric polypeptide.
17. An isolated polynucleotide encoding (a) a polypeptide that comprises the full-length amino acid sequence selected from the group consisting of SEQ ID NO:2, 8, 16, 55, and 78; or (b) a polypeptide that comprises an amino acid sequence at least 95% identical to the full-length amino acid sequence selected from the group consisting of SEQ ID NO:2, 8, 16, 55, and 78.
18. A vector comprising the isolated polynucleotide of claim 17 , wherein the polynucleotide is operably linked to an expression control region.
19. An isolated host cell transfected with the vector of claim 18 .
20. A process for producing a polypeptide, said process comprising culturing the host cell according to claim 19 under conditions suitable for expression of the polypeptide.