Genes of an otitis media isolate of nontypeable
View Patent ↗The invention relates to the polynucleotide sequence of a nontypeable stain of Haemophilus influenzae (NTHi) and polypeptides encoded by the polynucleotides and uses thereof. The invention also relates to NTHi genes which are upregulated during or in response to NTHi infection of the middle ear and/or the nasopharynx.
1. An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 599.
2. A method for detecting NTHi bacteria in a biological sample comprising
(a) contacting a polynucleotide of claim 1 with polynucleotides from a biological sample under stringent hybridization conditions, wherein the hybridization conditions comprise washing with 0.015 M sodium chloride, 0.0015 M sodium citrate at 65-68 ° C., and
(b) detecting hybridization of the polynucleotide within the sample, wherein hybridization indicates the presence of NTHi bacteria in the biological sample.
3. The method of claim 2 wherein the biological sample is selected from the group consisting of serum, sputum, ear fluid, blood, urine, lymphatic fluid, and cerebrospinal fluid.
4. A vector comprising the polynucleotide of claim 1 .
5. The vector of claim 4 , wherein the polynucleotide is operatively linked to an expression control sequence.
6. An isolated cell comprising the vector of claim 4 .
7. A host cell comprising the vector of claim 5 .
8. An isolated cell comprising the polynucleotide of claim 1 .