Method of screening a drug such as insulin secretagogue
The screening method of the present invention is useful for screening drugs such as insulin secretagogues having an insulin secretagogue activity with minimized side effects (hypoglycemia induction, etc.). The transformant in which a polynucleotide encoding the fusion protein used for the screening method is introduced, the screening kit comprising the transformant, etc. are also useful for screening excellent drugs.
1. A method of screening a substance regulating insulin secretion from a cell, which comprises:
contacting a cell transformed with a polynucleotide encoding a fusion protein of preproinsulin and a luciferase with a test substance, and
detecting luminescence of the luciferase to measure a level of insulin secretion,
wherein the luciferase is Gaussia luciferase, and
wherein Gaussia luciferase is a protein of any one of (a) through (c) below:
(a) a protein comprising the amino acid sequence of SEQ ID NO: 8;
(b) a protein consisting of an amino acid sequence comprising deletion, substitution, insertion and/or addition of 1 to 10 amino acid residues in the amino acid sequence of SEQ ID NO: 8, and having substantially the same activity as a protein consisting of the amino acid sequence of SEQ ID NO: 8;
(c) a protein consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 8, and having substantially the same activity as the protein consisting of the amino acid sequence of SEQ ID NO: 8; and,
wherein the preproinsulin consists of a signal peptide of preproinsulin and the polypeptide of any one of (i) through (k) below:
(i) a polypeptide consisting of the amino acid sequence of SEQ ID NO: 4;
(j) a polypeptide comprising a polypeptide consisting of an amino acid sequence comprising deletion, substitution, insertion and/or addition of 1 to 10 amino acid residues in the amino acid sequence of SEQ ID NO: 4, and having an insulin activity;
(k) a polypeptide comprising a polypeptide consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 4, and having an insulin activity; and,
wherein the signal peptide of preproinsulin is a polypeptide consisting of the amino acid sequence of SEQ ID NO: 6.
2. The screening method according to claim 1 , wherein the any one of (e) through (g) below:
(e) a polypeptide consisting of the amino acid sequence of SEQ ID NO: 10;
(f) a polypeptide comprising a polypeptide consisting of an amino acid sequence comprising deletion, substitution, insertion and/or addition of 1 to 10 amino acid residues in the amino acid sequence of SEQ ID NO: 10, and having an insulin activity;
(g) a polypeptide comprising a polypeptide consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 10, and having an insulin activity; and,
(h) a polypeptide comprising a polypeptide consisting of an amino acid sequence encoded by a polynucleotide that hybridizes under high stringent conditions to a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 9 wherein the high stringent conditions are 5×SSC, 5×Denhardt's solution, 0.5% (w/v) SDS, 50% (v/v) formamide and 50° C., and having an insulin activity.
3. The screening method according to claim 1 , wherein the fusion any one of (m) through (o) below:
(m) a polypeptide consisting of the amino acid sequence of SEQ ID NO: 12;
(n) a polypeptide comprising a polypeptide consisting of an amino acid sequence comprising deletion, substitution, insertion and/or addition of 1 to 10 amino acid residues in the amino acid sequence of SEQ ID NO: 12, and having an insulin activity;
(o) a polypeptide comprising a polypeptide consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 12, and having an insulin activity; and,
(p) a polypeptide comprising a polypeptide consisting of an amino acid sequence encoded by a polynucleotide that hybridizes under high stringent conditions to a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 11 wherein the high stringent conditions are 5×SSC, 5×Denhardt's solution, 0.5% (w/v) SDS, 50% (v/v) formamide and 50° C., and having an insulin activity.
4. The screening method according to claim 1 , which further comprises the step of detecting luminescence using a CCD camera or a photon counting camera.
5. A method of screening a substance regulating insulin secretion from a cell, which comprises:
contacting a cell transformed with a polynucleotide encoding a fusion protein of preproinsulin and a luciferase with a test substance, and
detecting luminescence of the luciferase to measure a level of insulin secretion,
wherein the luciferase is Gaussia luciferase, and
wherein Gaussia luciferase is a protein of any one of (a) through (c) below:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 8;
(b) a protein consisting of an amino acid sequence comprising deletion, substitution, insertion and/or addition of 1 to 5 amino acid residues in the amino acid sequence of SEQ ID NO: 8, and having substantially the same activity as a protein consisting of the amino acid sequence of SEQ ID NO: 8;
(c) a protein consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 8, and having substantially the same activity as the protein consisting of the amino acid sequence of SEQ ID NO: 8; and,
wherein the preproinsulin consists of a signal peptide of preproinsulin and the polypeptide of any one of (i) through (k) below:
(i) a polypeptide consisting of the amino acid sequence of SEQ ID NO: 4;
(j) a polypeptide comprising a polypeptide consisting of an amino acid sequence comprising deletion, substitution, insertion and/or addition of 1 to 10 amino acid residues in the amino acid sequence of SEQ ID NO: 4, and having an insulin activity;
(k) a polypeptide comprising a polypeptide consisting of an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 4, and having an insulin activity; and,
wherein the signal peptide of preproinsulin is a polypeptide consisting of the amino acid sequence of SEQ ID NO: 6.