Starches with high amylose content and improved processing properties
View Patent ↗The present invention relates to maize starches with an apparent amylose content between 35 wt. % and 90 wt. % and improved processing properties relative to conventional high-amylose maize starches. Furthermore, the present invention relates to maize meals and foodstuffs containing these maize starches or maize meals. In addition, the present invention relates to methods of production of said maize starches/maize meals and maize plants which synthesize these maize starches. Moreover, the present invention relates to wheat starches with an apparent amylose content between 35 wt. % and 90 wt. % and improved processing properties, and wheat meals and foodstuffs containing said wheat starches or wheat meals. Moreover, the present invention relates to methods of production of said wheat starches/wheat meals and wheat plants which synthesize these wheat starches.
1. An isolated maize plant cell comprising a heterologous GWD gene and an amylose extender mutation.
2. A maize plant comprising a maize plant cell as claimed in claim 1 .
3. The maize plant as claimed in claim 2 , wherein said maize plant synthesizes a maize starch that has an increased apparent amylose content relative to maize starch from a corresponding wild-type maize plant, and wherein said maize starch further displays a measurable pasting temperature in an aqueous suspension in RVA analysis, wherein the RVA analysis is measured according to the AACC standard method 61-02.
4. The maize plant as claimed in claim 3 , wherein the aqueous suspension is a 10% (w/v) aqueous suspension of the maize starch in water.
5. The maize plant as claimed in claim 3 , wherein the maize starch has a phosphate content in position C6 between 15 and 30 nmol C6P/mg starch (dry weight).
6. Reproductive material of the maize plant as claimed in claim 2 , wherein said reproductive material comprises the heterologous GWD gene and the amylose extender mutation.
7. Reproductive material of the maize plant as claimed in claim 3 , wherein said reproductive material comprises the heterologous GWD gene and the amylose extender mutation.
8. The maize plant cell of claim 1 , wherein said GWD gene comprises the coding region of the nucleic acid sequence of SEQ ID NO: 1.
9. The maize plant cell of claim 1 , wherein said GWD gene comprises the coding region of the nucleic acid sequence of SEQ ID NO: 3.
10. The maize plant cell of claim 1 , wherein said amylose extender mutation comprises a mutation in SEQ ID NO: 5.
11. The maize plant cell of claim 8 , further wherein said amylose extender mutation comprises a mutation in SEQ ID NO: 5.
12. The maize plant cell of claim 9 , further wherein said amylose extender mutation comprises a mutation in SEQ ID NO: 5.
13. A maize plant comprising the maize plant cell of claim 8 .
14. A maize plant comprising the maize plant cell of claim 9 .
15. A maize plant comprising the maize plant cell of claim 10 .
16. A maize plant comprising the maize plant cell of claim 11 .
17. A maize plant comprising the maize plant cell of claim 12 .
18. A method for the production of maize starch comprising extracting maize starch from the maize plant as claimed in claim 2 .
19. A method for the production of maize starch comprising extracting maize starch from the maize plant as claimed in claim 3 .
20. A method for the production of maize starch comprising extracting maize starch from the reproductive material as claimed in claim 6 .
21. A method for the production of maize meal comprising grinding the maize plant as claimed in claim 2 .
22. A method for the production of maize meal comprising grinding the maize plant as claimed in claim 3 .
23. A method for the production of maize meal comprising grinding the reproductive material as claimed in claim 6 .