Co-incubating confined microbial communities
This invention provides devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.
1. A method, comprising:
(1) providing an ingestible capsule with a defined spatial structure, the ingestible capsule comprising a first compartment and a second compartment connected to the first compartment via a communication channel, wherein the first and second compartments are physically separated by a distance x;
(2) placing a first individual type of microorganism in the first compartment;
(3) placing a second individual type of microorganism in the second compartment, wherein the distance x both reduces competition between the first and second individual types of microorganisms and allows for exchange of information and/or substance between the first and second individual types of microorganisms; and
(4) co-incubating the first individual type of microorganism in the first compartment and the second individual type of microorganism in the second compartment so that the first individual type of microorganism performs a function differently than it would if the second individual type of microorganism was absent.
2. The method of claim 1 , wherein during the co-incubating step, the first individual type of microorganism produces an incubation product that it otherwise would not produce when the second individual type of microorganism is absent.
3. The method of claim 1 , wherein during the co-incubating step, the first individual type of microorganism does not produce an incubation product that it otherwise would produce when the second individual type of microorganism is absent.
4. The method of claim 1 , wherein during the co-incubating step, the first individual type of microorganism degrades or removes an external component in an amount that differs from what it would otherwise degrade when the second individual type of microorganism is absent.
5. The method of claim 1 , wherein a chemical and/or biological substance is transported through the communication channels between the different individual type of microorganisms placed in the first compartment and the second compartment.
6. The method of claim 1 , wherein the ingestible capsule further comprises a barrier disposed in the communication channel, wherein the barrier blocks passage of microorganisms from one compartment to another, but allows a soluble chemical and/or biological substance to pass from one compartment to another.
7. The method of claim 6 , wherein the barrier is selected from a group consisting of a membrane, a gel, a nanostructure, a pore, and a structure that is not permeable to microorganisms but is permeable to the soluble chemical and/or biological substance.
8. The method of claim 1 , wherein the distance x is about 25 microbe lengths, the microbe length defined by the average length of the first and second individual type of microorganisms.
9. The method of claim 1 , wherein the distance x is between about 50 μm and about 500 μm.
10. The method of claim 1 , wherein the first and second individual types of microorganisms are environmentally incompatible.
11. The method of claim 1 , wherein the first and second individual types of microorganisms are localized in the first and second compartments, respectively.
12. The method of claim 1 , wherein the second individual type of microorganism performs a function differently than it would if the first individual type of microorganism was absent.