Method for prenatal diagnosis
View Patent ↗The invention discloses a method of prenatal diagnosis comprising the step of isolating exosomes from an isolated fluid, wherein the exosomes are identified by biomarker detection. Furthermore, the invention discloses the isolation of exosomes from an isolated fluid and the use of a biomarker, particularly CD24 to isolate exosomes from an isolated fluid.
1. A method for detecting the presence of exosomes in an isolated fluid comprising identifying the exosomes in the isolated fluid by detecting the presence of CD24-marker on the exosomes.
2. The method of claim 1 , wherein the exosomes are fetus-derived exosomes.
3. The method of claim 1 , wherein the exosomes are tumor-derived exosomes.
4. The method of claim 3 , wherein at least one further biomarker is detected, said at least one further biomarker being selected from the group consisting of: Annexin-1, EpCAM, ADAM10, CD9 and the cell adhesion molecule L1.
5. The method of claim 3 , wherein the presence of tumor-derived exosomes is indicative for malignant ascites.
6. A method for prenatal diagnosis to detect congenital anomalies of a fetus comprising the steps of:
(a) isolating exosomes from an isolated fluid sample, and
(b) detecting pathological fetal conditions from the exosomes isolated in the fluid sample;
wherein (i) the exosomes are identified by biomarker detection, (ii) the biomarker is CD24, (iii) the isolated fluid sample containing the exosomes is derived from or originated from the fetus, and (iv) the CD24-positive exosomes carry fetal genetic information which provides prenatal diagnosis of congenital anomalies in the fetus.
7. The method of claim 6 , wherein the isolated fluid sample is one of the group consisting of amniotic fluid, urine and serum.
8. The method of claim 6 , wherein said exosomes are fetus-derived exosomes.
9. An isolated exosome, wherein the exosome comprises a CD24-marker.
10. The exosome of claim 9 , wherein the exosome is obtainable by a method comprising the steps of isolating exosomes from an isolated fluid sample and identifying the exosomes by CD24-marker detection.