Methods of making monoclonal antibodies using fusion-peptide epitope adoptive transfer (F-PEAT) technology
Disclosed are compositions and methods for related to monoclonal antibodies specific for single amino acid variation in an antigen.
1. A method of making a monoclonal antibody comprising the steps of
a. administering to a subject CD4 T cells from a donor specific for a first antigen, wherein said CD4 T cells are of the same species as the subject and the donor and subject are MHC matched;
b. administering to a subject a cell comprising a chimeric antigen wherein the chimeric antigen comprises a first antigen linked to the terminal end of a second antigen, wherein the subject expresses a third antigen and wherein the third antigen differs from the second antigen by a single amino acid variation; and
c. generating a monoclonal antibody specific to the second antigen.
2. The method of claim 1 , wherein the subject is a mammal.
3. The method of claim 2 , wherein the mammal is a mouse.
4. The method of claim 1 , wherein the subject is transgenic for the third antigen.
5. The method of claim 1 , wherein the subject is tolerized to the third antigen.
6. The method of claim 1 , wherein the first antigen is a CD4 T cell epitope.
7. The method of claim 1 , wherein the CD4 T cells are isolated from a second subject comprising T-cell receptor (TCR) transgene specific for the first antigen.
8. The method of claim 1 , wherein the CD4 T cells are derived from a CD4 T cell line.
9. The method of claim 1 , wherein the second antigen is a blood antigen.
10. The method of claim 9 , wherein the blood antigen is selected from the listing of blood antigens consisting of Duffy A, Duffy B, Kell antigen K 1 , Kell antigen K 2 , S antigen, H substance, Yt(a), and Yt(b).