Tetranor PGDM: a biomarker of PGD
The present invention relates to a prostaglandin D 2 metabolite, derivatives thereof, compositions comprising the metabolite, and an antibody that specially binds to the metabolite. Methods of use are also provided.
1. A method of detecting whether prostaglandin D 2 (PGD 2 ) has been metabolized in an animal, the method comprising the steps of:
providing a biological sample from an animal, and
detecting an underivatized tetranor PGDM of Formula I in the biological sample using a method comprising liquid chromatography/tandem mass spectroscopy or an immunoassay
wherein, if the underivatized tetranor PGDM of formula I is detected in the biological sample of the animal, PGD 2 has been metabolized in the animal.
2. The method of claim 1 , further comprising isolating the underivatized tetranor PGDM of formula I from the sample.
3. The method of claim 1 , wherein the biological sample is urine.
4. The method of claim 2 , further comprising quantifying the amount of the isolated underivatized tetranor PGDM in the sample.