Boundary conditions for the arrangement of cells and tissues
The present invention relates to the arrangement of one or more cells in a medium or on a substrate through the use of boundary conditions, which are changes in local environment compared to the medium or substrate alone or cause an alteration of cell response upon interaction of a cell with the boundary condition.
1. An in vitro method of forming a mature muscle tissue, comprising:
(a) providing a medium or substrate;
(b) providing a boundary condition associated with the medium or substrate, wherein said boundary condition directs the alignment and development of dissociated muscle cells to form a mature muscle tissue;
(c) providing the dissociated muscle cells to the medium or substrate, wherein the muscle cells arrange based upon the boundary condition; and
(d) culturing the muscle cells to form a mature muscle tissue,
wherein the boundary condition directs the movement and growth of the muscle cells upon interaction with the boundary condition as compared to interaction with the medium or substrate alone such that substantially all of the muscle cells and sarcomeres within the muscle cells are spatially anisotropic in at least one direction and substantially all of the muscle cells coordinately contract, thereby forming a mature muscle tissue.
2. An in vitro method of forming a mature muscle tissue, comprising:
(a) providing a medium or substrate;
(b) providing a boundary condition associated with the medium or substrate, wherein said boundary condition directs the alignment and development of dissociated muscle cells to form a mature muscle tissue;
(c) providing the muscle cells to the medium or substrate, wherein the muscle cells arrange based upon the boundary condition; and
(d) culturing the dissociated muscle cells to form a mature muscle tissue,
wherein the boundary condition is a change in local environment as compared to the environment of the medium or substrate alone and directs the movement and growth of the muscle cells such that substantially all of the muscle cells and sarcomeres within the muscle cells are spatially anisotropic in at least one direction and substantially all of the muscle cells coordinately contract, thereby forming a mature muscle tissue.
3. The method of claim 1 or 2 , wherein the boundary condition is a naturally-provided boundary condition.
4. The method of claim 1 or 2 , wherein the substrate is comprised of a polymeric material.
5. The method of claim 1 or 2 , wherein the boundary condition is selected from the group consisting of a physical, a mechanical, a chemical, and an electromagnetic boundary condition.
6. The method of claim 1 or 2 , wherein the boundary condition is a biochemical boundary.
7. The method of claim 6 , wherein the biochemical boundary comprises fibronectin.
8. The method of claim 1 or 2 , wherein the boundary condition is a physical boundary.
9. The method of claim 8 , wherein the physical boundary provides elasticity, surface roughness, or surface topography.
10. The method of claim 8 , wherein the physical boundary comprises micro- or nano-fabricated structures, said structures selected from the group consisting of ridges, pillars, and grooves.