Composition for promoting cartilage formation or repair comprising a NELL gene product and method of treating cartilage-related conditions using such composition
View Patent ↗Provided herein are a composition for promoting cartilage formation or regeneration comprising a NELL gene product and a method of treating cartilage-related conditions using such a composition.
1. A composition for promoting cartilage formation or repair, comprising a NELL peptide, wherein the NELL peptide is in an effective amount for cartilage formation or repair,
wherein the NELL peptide comprises SEQ ID NO: 2, 4, or 6 or a fragment of SEQ ID NO: 2, 4, or 6; and
wherein the composition is in an implantable formulation so as to promote cartilage formation or repair.
2. The composition of claim 1 , further comprising a second agent.
3. The composition of claim 1 , further comprising a second agent selected from the group consisting of chondroprotective agents, anti-pain and/or anti-inflammatory agents, growth factors, cytokines, small molecules, anti-angiogenic factors and combinations thereof.
4. The composition of claim 1 , further comprising a pharmaceutically acceptable carrier.
5. The composition of claim 2 , further comprising a pharmaceutically acceptable carrier.
6. The composition of claim 1 , further comprising a material that comprises a chemical gel, a physical gel, an interpenetrating network, or a crosslinker.
7. The composition of claim 1 in an injectable or moldable formulation that sets upon application to a site in a body part of a human being.
8. The composition of claim 1 , further comprising a material that degrades or releases the NELL peptide in response to a stimulus.
9. The composition of claim 8 , wherein the stimulus is selected from mechanical stimuli, light, electromagnetic field, temperature changes, pH changes, or changes of ionic strength.
10. The composition of claim 1 , further comprising an osteochondroprogenitor cell.
11. The composition of claim 1 , further comprising an osteochondroprogenitor cell selected from mesenchymal cells, fetal embryonic cells, stem cells, bone marrow cells, adipose stem cells, fibroblasts, or combinations thereof.
12. The composition of claim 1 , further comprising a chondrogenic cell.
13. The composition of claim 1 , wherein cartilage formation or repair is formation or repair of hyaline or tracheal cartilage, elastic cartilage, or fibrocartilage.
14. The composition of claim 1 , formulated into a device.
15. The composition of claim 1 , wherein the NELL peptide is in an amount effective for treating, or ameliorating a cartilage related disorder.
16. The composition of claim 15 , wherein the cartilage related disorder is arthropathies of various joints, arthritis of various joints, internal cartilage derangements of various joints, or spinal joint and disc-related disorders.
17. An implant for use in the human body comprising a substrate having a surface, wherein at least a portion of the surface includes a composition according to claim 1 .
18. An implant for use in the human body comprising a substrate having a surface, wherein at least a portion of the surface includes a composition according to claim 2 .
19. An implant for use in the human body comprising a substrate having a surface, wherein at least a portion of the surface includes a composition according to claim 3 .
20. An implant for use in the human body comprising a substrate having a surface, wherein at least a portion of the surface includes a composition according to claim 4 .
21. An implant for use in the human body comprising a substrate having a surface, wherein at least a portion of the surface includes a composition according to claim 5 .
22. An implant for use in the human body comprising a substrate having a surface, wherein at least a portion of the surface includes a composition according to claim 10 .
23. The implant of claim 17 , wherein the substrate is resorbable.
24. The implant of claim 17 , wherein the substrate comprises collagen.
25. The implant of claim 18 , wherein the substrate is resorbable.
26. The implant of claim 18 , wherein the substrate comprises collagen.
27. The implant of claim 17 , which is a device selected from:
an injectable/implantable device containing NELL protein with or without cells that can be directly injected or implanted into a spinal disc to promote cartilage formation;
a disc nucleus replacement device impregnated with NELL that is designed to replace the inner portion of a vertebral disc or both the inner and outer portion of a vertebral disc;
an injectable/implantable device containing NELL with or without cells that can be directly injected into various joint spaces or implanted arthroscopically or openly into various joint spaces;
an injectable/implantable device containing NELL protein with or without cells and other factors that can be directly injected/implanted into a spinal disc to promote cartilage formation;
a disc nucleus replacement device impregnated with NELL and other factors that is designed to replace the inner portion of a vertebral disc or both the inner and outer portion of a vertebral disc; or
an injectable/implantable device containing NELL and other factors with or without cells that can be directly injected into various joint spaces or implanted arthroscopically or openly into various joint spaces.