NANOSCALE MOLECULE SYNTHESIS
This invention relates to nanoscale molecule synthesis, including three-dimensional addressable arrays of biopolymeric nucleic acids and processes for manufacturing such arrays. Such arrays can be functionalized with complementary chemical reactive probes to provide catalytic enzymes.
1 . A method for enabling the connection of additional elements or molecules to a polynucleobase biopolymer weave comprising incorporating additional chemical linkages within the polynucleobase biopolymer weave.
2 . A method according to claim 1 , wherein at least one additional chemical linkage enables amide coupling.
3 . A method according to claim 1 , wherein at least one additional chemical linkage enables cycloaddition.
4 . A method according to claim 1 , wherein at least one additional chemical linkage enables ester coupling.
5 . A method according to claim 1 , wherein at least one additional chemical linkage enables disulfide linking.
6 . A method according to claim 1 , wherein the polynucleobase biopolymer weave comprises a nucleic acid.
7 . A method according to claim 6 , wherein the nucleic acid comprises PNA (peptide nucleic acid).
8 . A method according to claim 6 , wherein the nucleic acid comprises DNA (deoxyribonucleic acid).
9 . A method according to claim 6 , wherein the nucleic acid comprises RNA (ribonucleic acid).
10 . A method according to claim 6 , wherein the nucleic acid comprises LNA (locked nucleic acid).
11 . A method according to claim 6 , wherein the nucleic acid comprises GNA (glycol nucleic acid).
12 . A method according to claim 6 , wherein the nucleic acid comprises TNA (threose nucleic acid).
13 . A method of generating polynucleobase derivatives, comprising providing an additional chemical linkage aside from the Watson-Crick base pairing link or the backbone link.
14 . A method according to claim 13 , wherein the polynucleobase comprises thymine.
15 . A method according to claim 13 , wherein the polynucleobase comprises uracil.
16 . A method according to claim 13 , wherein the polynucleobase comprises cytosine.
17 . A method according to claim 13 , wherein the polynucleobase comprises guanine.
18 . A method according to claim 13 , wherein the polynucleobase comprises adenine.
19 . A method according to claim 1 , wherein the molecules are placed in relation to each other with tolerances of less than about 10 nanometers.
20 . A method of generating a catalyst, comprising using the method of claim 1 to generate a catalyst mimicking known enzymatic transformations but having superior properties to the analogous natural system regarding stability toward pH, temperature, the presence of additives known to accelerate catalysis, or a combination thereof.
21 . A method of generating an electrical circuit, comprising using the method of claim 1 to produce a 3D addressable assemblies of polynucleobases with feature size less than about 1/16 of the feature size currently amenable to scaled fabrication by photolithographic techniques.