Functionalized polydiacetylene sensors
View Patent ↗A microarray includes a solid substrate having a surface, the surface having a plurality of binding spots and a plurality of reaction moieties bound to the binding spots. A reaction moiety includes a plurality of polyacetylene monomers, the polyacetylene monomers having a first coupling region and a second coupling region, the first coupling region having a first functional group operable to bind to the binding spot and the second coupling region comprising a second functional group operable to bind to an accessory molecule; and an accessory molecule having a binding region and an analyte reaction region, the analyte reaction region operable to selectively bind to the target analyte, and the binding region operable to bind to the second coupling region of the polyacetylene monomer. Upon binding a target analyte with the reaction moiety, a color change from the polyacetylene monomer occurs and the reaction moiety produces fluorescence.
1. A polydiacetylene (PDA) monomer comprising the formula:
wherein,
R 1 is an alkyl group comprising about twelve carbon atoms;
R 2 is an alkyl group comprising about eight carbon atoms; and
R 3 is
2. The PDA monomer of claim 1 , wherein the monomer is coupled to an affinity component using the epoxy group.
3. The PDA monomer of claim 2 , wherein the affinity component has affinity for potassium and comprises a polynucleotide of SEQ ID NO: 1.
4. The PDA monomer of claim 2 , wherein the affinity component has affinity for mercury and comprises a polynucleotide of SEQ ID NO: 2.
5. The PDA monomer of claim 2 , wherein the affinity component is selected from the group consisting of a chemical ligand, antibody, antibody fragment, oligonucleotide, antigen, polypeptide, glycolipid, protein, enzyme, peptide nucleic acid, and polysaccharide.
6. A liposome comprising a plurality of PDA monomers according to claim 1 .
7. A microarray comprising a substrate comprising a plurality of discrete locations wherein each location comprises a plurality of PDA monomers according to claim 1 .
8. The microarray of claim 7 , wherein the plurality of PDA monomers in at least two discrete locations is coupled to different affinity components using the epoxy group.
9. The microarray of claim 7 , wherein the plurality of PDA monomers is in the form of a liposome or a wire.
10. The microarray of claim 9 , wherein the liposome is coupled to the discrete locations on the substrate using the epoxy group.
11. A PDA monomer comprising:
R 1 —C≡C—C≡C—R 2 —R 4
wherein,
R 1 is an alkyl group of about twelve carbon atoms;
R 2 is an alkyl group of about eight carbon atoms; and
R 4 is a member of the group consisting of:
12. A PDA monomer comprising:
R 1 —C≡C—C≡C—R 2 —R 5
wherein,
R 1 is an alkyl group of about twelve carbon atoms;
R 2 is an alkyl group of about eight carbon atoms; and
R 5 is a member of the group consisting of: