Plant cells and plants which synthesize a starch with an increased final viscosity
View Patent ↗The present invention relates to a plant cell which is genetically modified, the genetic modification leading to the reduction of the activity of one or more SSIII proteins which occur endogenously in the plant cell and to the reduction of the activity of one or more BEI proteins which occur endogenously in the plant cell and to the reduction of the activity of one or more BEII proteins which occur endogenously in the plant cell in comparison with corresponding plant cells, of wild-type plants, which have not been genetically modified. Further aspects of the invention relate to plants containing such plant cells, to a method for generating the plant cells and plants, and to the starch obtainable from them.
1. A method for producing a starch comprising extracting starch from a genetically modified dicotyledonous plant cell, wherein said genetically modified plant cell comprises
(1) a first foreign nucleic acid molecule, wherein said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1;
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4; and
(3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6 and
further wherein said genetically modified plant cell synthesizes a modified starch, which after gelatinization of a 6% suspension in water forms a gel with a gel strength that is increased by at least 300% in comparison with the gel strength of starch extracted from corresponding plant cells, of wild-type plants, which have not been genetically modified.
2. A method for producing a starch, comprising extracting starch from a genetically modified plant comprising a genetically modified dicotyledonous plant cell, wherein said genetically modified plant cell comprises
(1) a first foreign nucleic acid molecule, wherein said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1;
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4; and
(3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6, and
further wherein said genetically modified plant cell synthesizes a modified starch, which after gelatinization of a 6% suspension in water forms a gel with a gel strength that is increased by at least 300% in comparison with the gel strength of starch extracted from corresponding plant cells, of wild-type plants, which have not been genetically modified.
3. The method of claim 1 , wherein said plant cell is a potato plant cell.
4. The method of claim 1 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6.
5. The method of claim 1 , wherein
(1) said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1;
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4; and
(3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6.
6. The method of claim 5 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6.
7. The method of claim 2 , wherein said plant cell is a potato plant cell.
8. The method of claim 2 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 95% identity with SEQ ID NO: 6.
9. The method of claim 2 , wherein
(1) said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1;
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4; and
(3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6.
10. The method of claim 9 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising SEQ ID NO: 6.
11. A method for producing a starch comprising extracting starch from a genetically modified dicotyledonous plant cell, wherein said genetically modified plant cell comprises
(1) a first foreign nucleic acid molecule, wherein said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1;
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4; and
(3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6, and
further wherein said genetically modified plant cell synthesizes a modified starch, which after gelatinization of a 6% suspension in water forms a gel with a gel strength that is increased by at least 300% in comparison with the gel strength of starch extracted from corresponding plant cells, of wild-type plants, which have not been genetically modified.
12. The method of claim 11 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6.
13. The method of claim 11 , wherein
(1) said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1.
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4; and
(3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6.
14. The method of claim 13 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6.
15. The method of claim 11 , wherein said plant cell is a potato plant cell.
16. A method for producing a starch comprising extracting starch from a genetically modified plant comprising a genetically modified dicotyledonous plant cell, wherein said genetically modified plant cell comprises
(1) a first foreign nucleic acid molecule, wherein said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1;
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4; and (3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6, and
further wherein said genetically modified plant cell synthesizes a modified starch, which after gelatinization of a 6% suspension in water forms a gel with a gel strength that is increased by at least 300% in comparison with the gel strength of starch extracted from corresponding plant cells, of wild-type plants, which have not been genetically modified.
17. The method of claim 16 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 100 nucleotides of SEQ ID NO: 6.
18. The method of claim 16 , wherein
(1) said first foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1,
(2) a second foreign nucleic acid molecule, wherein said second foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4; and
(3) a third foreign nucleic acid molecule, wherein said third foreign nucleic acid molecule is
(a) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6;
(b) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6; or
(c) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6.
19. The method of claim 18 , wherein
said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 1;
said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 4; and
said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEII protein, said DNA molecule comprising at least 500 nucleotides of SEQ ID NO: 6.
20. The method of claim 16 , wherein said plant cell is a potato plant cell.