Identification of the gene and mutation responsible for progressive rod-cone degeneration in dog and a method for testing same
Tools and methods are provided for determining whether or not a dog is genetically normal, is a carrier of, or is affected with or predisposed to progressive rod-cone degeneration. The method is based on the detection of a transversion from G to A at position corresponding to nucleotide position 1298 of SEQ ID NO: 1.
1. A method for identifying a dog as genetically normal, as a carrier of, or as affected with or predisposed to progressive rod cone degeneration comprising:
a) providing a biological sample obtained from the dog, said biological sample comprising nucleic acids; and
b) detecting in the biological sample whether or not there is a change from G to A at position 1298 in SEQ ID NO:1, identifying the dog to be a carrier of progressive rod cone degeneration, affected with or predisposed to progressive rod cone degeneration, or normal, wherein the G to A change in one allele is indicative of a carrier of progressive rod-cone degeneration, the G to A change in both alleles is indicative of a dog affected with or predisposed to progressive rod-cone degeneration, and the absence of the G to A change is indicative of a genetically normal dog.
2. The method of claim 1 , wherein the nucleic acid is DNA.
3. The method of claim 1 , wherein the nucleic acid is mRNA.
4. The method of claim 1 , wherein the biological sample is any tissue containing genomic DNA or mRNA.
5. The method of claim 1 , wherein the biological sample is selected from the group consisting of blood, hair, mucosal scrapings, semen, tissue biopsy and saliva.
6. The method of claim 1 , wherein the biological sample is saliva.
7. A method for identifying a dog as genetically normal, as a carrier of, or as affected with or predisposed to progressive rod cone degeneration comprising:
a) providing a biological sample obtained from the dog, said biological sample comprising nucleic acids;
b) detecting in the biological sample whether or not there is a change from G to A at position 1298 in SEQ ID NO:1;
c) based on the detecting in b), identifying the dog to be a carrier of progressive rod cone degeneration, affected with or predisposed to progressive rod cone degeneration, or normal, wherein the G to A change in one allele is indicative of a carrier of progressive rod-cone degeneration, the G to A change in both alleles is indicative of a dog affected with or predisposed to progressive rod-cone degeneration, and the absence of the G to A change is indicative of a genetically normal dog.
8. The method of claim 7 , wherein the nucleic acid is DNA.
9. The method of claim 7 , wherein the nucleic acid is mRNA.
10. The method of claim 7 , wherein the biological sample is any tissue containing genomic DNA or mRNA.
11. The method of claim 10 , wherein the biological sample is selected from the group consisting of blood, hair, mucosal scrapings, semen, tissue biopsy and saliva.
12. The method of claim 11 , wherein the biological sample is saliva.