PERFUSION DETECTION DEVICES AND METHODS OF USING THE SAME
Hand-held pocket-sized self-contained perfusion detection devices are provided. Devices of the invention are configured to rapidly provide a perfusion detection result without the need for pre-calibration. Also provided are methods of using the perfusion detection devices, as well as kits that include the devices and/or components thereof for use in practicing such methods.
1 . A hand-held perfusion detection device, said device comprising:
a power source;
a light source;
a detector; and
a processor configured to provide a perfusion detection result in 1 minute or less following illumination of a target tissue site with light from the light emitting diode without pre-calibration;
wherein the power source, light emitting diode, detector and processor are present in a self-contained pocket-sized housing.
2 . The device according to claim 1 , wherein the processor is configured to provide a perfusion detection result substantially immediately.
3 . The device according to claim 2 , wherein the light source is a light emitting diode.
4 . The device according to claim 1 , wherein the detector is a photodiode.
5 . The device according to claim 4 , wherein the device comprises a signal amplifier coupled to the photodiode.
6 . The device according to claim 5 , wherein the signal amplifier and photodiode are present in an EMI shield.
7 . The device according to claim 1 , wherein the device comprises a wave generator configured to provide a pulse-wave signal to the light emitting diode.
8 . The device according to claim 7 , wherein the device comprises a lock-in amplifier configured to receive a signal from the detector.
9 . The device according to claim 8 , wherein the lock-in amplifier is configured to receive a pulse-wave reference signal from the wave generator.
10 . The device according to claim 8 , wherein the device comprises a root mean square converter configured to receive a signal from the lock-in amplifier.
11 . The device according to claim 10 , wherein the device comprises low pass filter configured to receive a signal from the root mean square converter.
12 . The device according to claim 11 , wherein the processor comprises an analog to digital signal converter configured to receive a signal from the low pass filter and output a digital signal to a functional block that converts the digital signal to the perfusion detection result.
13 . The device according to claim 12 , wherein the processor further comprises the wave generator.
14 . The device according to claim 13 , wherein the device comprises a display configured to display the perfusion detection result.
15 . The device according to claim 1 , wherein the device comprises an interchangeable filter operably positioned relative to the detector.
16 . The device according to claim 1 , wherein the light emitting diode is interchangeable.
17 . The device according to claim 1 , wherein the processor comprises a wave generator having a frequency that is programmably adjustable.
18 . The device according to claim 17 , wherein the frequency can be programmably adjusted to frequency ranging from 1 Hz to 1 MHz.
19 . The device according to claim 1 , wherein the power source is a battery.
20 . The device according to claim 1 , wherein the housing comprises a body section and head section.
21 . The device according to claim 20 , wherein the head section is movable relative to the body section.
22 . The device according to claim 21 , wherein the head section comprises the detector and the body section comprises the power source, light emitting diode and processor.
23 . The device according to claim 22 , wherein the device is configured to emit and receive light through a common aperture.
24 . The device according to claim 23 , wherein the device comprises an ambient light shield configured to shield the detector from ambient light.
25 . The device according to claim 24 , wherein the ambient light shield is a speculum.
26 . A method comprising:
administering a fluorescent agent to the subject; and
obtaining a perfusion detection result for target tissue from a hand-held perfusion detection device comprising:
a power source;
a light emitting diode;
a detector; and
a processor configured to provide a perfusion detection result in 1 minute or less following illumination of a target tissue site with light from the light emitting diode without pre-calibration;
wherein the power source, light emitting diode, detector and processor are present in a self-contained pocket-sized housing.
27 . The method according to claim 26 , wherein the fluorescent agent is administered to the subject by injection.
28 . The method according to claim 26 , wherein the fluorescent agent is fluorescein.
29 . The method according to claim 16 , wherein the perfusion detection result is obtained in 1 minute or less following illumination of the target tissue with the light emitting diode.
30 . The method according to claim 29 , wherein the perfusion detection result is obtained in 5 seconds or less following illumination of the target tissue with the light emitting diode.
31 . A kit comprising:
(a) a hand-held perfusion detection device, said device comprising:
a power source;
a light emitting diode;
a detector; and
a processor configured to provide a perfusion detection result in 1 minute or less following illumination of a target tissue site with light from the light emitting diode without pre-calibration;
wherein the power source, light emitting diode, detector and processor are present in a self-contained pocket-sized housing; and
(b) instructions for using the device in a perfusion detection application.
32 . The kit according to claim 31 , wherein the kit further comprises a dosage of a fluorescent agent.
33 . The kit according to claim 31 , wherein the kit further comprises two or more light emitting diodes of differing light emission properties.
34 . The kit according to claim 33 , wherein the kit further comprises two or more differing optical filters configured to be operably positioned in the device relative to the detector.