Methods and compositions comprising capture agents
View Patent ↗The present invention relates to methods and compositions for detecting a substance using mixed or multiple element capture agents (MECA). The affinity of a MECA for a target is produced by the concomitant binding of at least two low to moderate affinity capture agents providing a high affinity interaction with a capture target.
1. An article comprising a mixed element capture agent (MECA) for assessing the presence of at least a first target molecule in a sample comprising a plurality of low-to-moderate affinity binding elements distributed on a surface of, and coupled to a support, wherein concomitant binding of the first target molecule by a first and second binding element results in a high affinity interaction with the first target molecule.
2. The article of claim 1 , wherein the binding elements are peptides, peptoids (N-substituted oligoglycines) or other peptide-like oligomers.
3. The article of claim 1 , wherein the first and a second binding element have distinct binding specificity for a target molecule as compared to each other.
4. The article of claim 1 , wherein a first binding element is covalently coupled to the second binding element forming a chimeric binding element.
5. The article of claim 4 , wherein the chimeric binding element comprises a spacer coupled to the first binding element, the second binding element or both the first and second binding element.
6. The article of claim 5 , wherein the second binding element is an oligomer.
7. The article of claim 6 , wherein the oligomer is a peptide or peptide derivative.
8. The article of claim 7 , wherein the peptide derivative is comprised of one or more non-natural amino acid.
9. The article of claim 7 , wherein the peptide derivative comprises one or more peptoid monomers.
10. The article of claim 1 , wherein the sample is an environmental sample, a cell lysate, a blood sample, a sputum sample or a urine sample.
11. The article of claim 1 , wherein the binding elements are distributed randomly on the surface of the support.
12. The article of claim 1 , further comprising at least a third and a fourth low-to-moderate binding element that bind a second target molecule, the third and fourth binding element distributed on a surface of, and operatively coupled to, the support, wherein concomitant binding of the second target molecule to the third and fourth binding elements results in a high affinity interaction with the second target molecule.
13. A method of determining the presence of a target molecule in a sample comprising:
a) exposing the sample to a mixed element capture agent comprising a plurality of low-to-moderate affinity binding elements distributed on a surface of, and operatively coupled to a support, wherein concomitant binding of the target molecule to at least a two of the low-to-moderate affinity binding elements results in a specific high affinity interaction with the target molecule; and
b) evaluating binding of the target molecule to the binding elements.