Method and system for modeling cellular metabolism
This invention relates to methods and systems for in silico or bioinformatic modeling of cellular metabolism. The invention includes methods and systems for modeling cellular metabolism of an organism, comprising constructing a flux balance analysis model, and applying constraints to the flux balance analysis model, the constraints selected from the set consisting of: qualitative kinetic information constraints, qualitative regulatory information constraints, and differential DNA microarray experimental data constraints. In addition, the present invention provides for computational procedures for solving metabolic problems.
1. A method for determining a reduced genome, comprising:
providing a set of metabolic reactions;
forming an optimization problem in a suitably programmed computer having a stoichiometric model of metabolism;
applying binary logic variables to associate gene activations and gene inactivations with the metabolic reactions and simultaneously solving the optimization problem to determine the effect of the set of metabolic reactions on a growth rate target;
selecting a minimal set of reactions from the set of metabolic reactions that meets the growth rate target; and
mapping enzymes catalyzing the minimal set of reactions to a corresponding set of coding genes to determine a reduced genome, and providing an output to a user of said reduced genome.
2. The method of claim 1 wherein the growth rate target is a biomass target production rate.
3. The method of claim 2 , wherein the biomass target production rate is selected from the group consisting of 10%, 60%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5% and 100% of 100% biomass generation.
4. The method of claim 1 , wherein the growth rate target is selected from the group consisting of 1%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% and 100% of maximum growth rate.
5. The method of claim 4 , wherein the metabolic component is a metabolite.
6. The method of claim 1 , wherein the growth rate target is a selected level of ATP production.
7. The method of claim 1 , wherein the growth rate target is a selected level of metabolic components.